Exploration of Chemical Diversity in Intercellular Quorum Sensing Signalling Systems in Prokaryotes

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorJonkergouw, Christopheren_US
dc.contributor.authorSavola, Pihlaen_US
dc.contributor.authorOsmekhina, Ekaterinaen_US
dc.contributor.authorvan Strien, Joerien_US
dc.contributor.authorBatys, Piotren_US
dc.contributor.authorLinder, Markus B.en_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorBiomolecular Materialsen
dc.contributor.groupauthorCenter of Excellence in Life-Inspired Hybrid Materials, LIBERen
dc.contributor.organizationRadboud University Nijmegenen_US
dc.contributor.organizationInstitute of Catalysis and Surface Chemistry of the Polish Academy of Sciencesen_US
dc.date.accessioned2024-01-17T08:28:22Z
dc.date.available2024-01-17T08:28:22Z
dc.date.issued2024-01-08en_US
dc.descriptionFunding Information: The authors would like to acknowledge funding from the Academy of Finland through its Centre of Excellence Programme Life‐Inspired Hybrid Materials (LIBER 2022–2029, grant 346105: C.J., E.O., M.B.L.) and Academy Projects (grant 272578: C.J., E.O., M.B.L.). P.B. would like to thank the European Union Erasmus+Programme (project no: 2019‐1‐PL01‐KA103‐061592) for providing financial support for the mobility and training in Aalto University. We gratefully acknowledge Poland's high‐performance computing infrastructure PLGrid (HPC Centers: ACK Cyfronet AGH) for providing computer facilities and support within computational grant no. PLG/2023/016229. We would like to thank Professor Helge B. Bode and Peter Grün, for providing the QS ligands DAR IPS, PPYA and PPYB. We would like to thank Prof. Ralf Heermann, for the sharing of plasmids containing PluR, PauR and promoter sequences. Furthermore, we would like to thank the Coil Genetic Stock Center (CGSC) for the Keio knockout strains. Finally, we would like to thank Dr. Georg Schmidt, Meryem Ecem Kaya and Saleh Khan for the input and discussions. E. coli Publisher Copyright: © 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
dc.description.abstractQuorum sensing (QS) serves as a vital means of intercellular signalling in a variety of prokaryotes, which enables single cells to act in multicellular configurations. The potential to control community-wide responses has also sparked numerous recent biotechnological innovations. However, our capacity to utilize intercellular communication is hindered due to a scarcity of complementary signalling systems and a restricted comprehension of interconnections between these systems caused by variations in their dynamic range. In this study, we utilize uniform manifold approximation and projection and extended-connectivity fingerprints to explore the available chemical space of QS signalling molecules. We investigate and experimentally characterize a set of closely related QS signalling ligands, consisting of N-acyl homoserine lactones and the aryl homoserine lactone p-coumaroyl, as well as a set of more widely diverging QS ligands, consisting of photopyrones, dialkylresorcinols, 3,5-dimethylpyrazin-2-ol and autoinducer-2, and define their performance. We report on a set of six signal- and promoter-orthogonal intercellular QS signalling systems, significantly expanding the toolkit for engineering community-wide behaviour. Furthermore, we demonstrate that ligand diversity can serve as a statistically significant tool to predict much more complicated ligand-receptor interactions. This approach highlights the potential of dimensionality reduction to explore chemical diversity in microbial dynamics.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationJonkergouw, C, Savola, P, Osmekhina, E, van Strien, J, Batys, P & Linder, M B 2024, ' Exploration of Chemical Diversity in Intercellular Quorum Sensing Signalling Systems in Prokaryotes ', Angewandte Chemie - International Edition, vol. 63, no. 2, e202314469 . https://doi.org/10.1002/anie.202314469en
dc.identifier.doi10.1002/anie.202314469en_US
dc.identifier.issn1433-7851
dc.identifier.otherPURE UUID: b2172f98-e2f3-4c9a-bffc-d0a51b6a47cfen_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/b2172f98-e2f3-4c9a-bffc-d0a51b6a47cfen_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85176090131&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/133415692/CHEM_Jonkergouw_et_al_Exploration_of_Chemical_2024_Angewandte_Chemie.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/125858
dc.identifier.urnURN:NBN:fi:aalto-202401171533
dc.language.isoenen
dc.publisherWiley-VCH Verlag
dc.relation.ispartofseriesAngewandte Chemie - International Editionen
dc.relation.ispartofseriesVolume 63, issue 2en
dc.rightsopenAccessen
dc.subject.keywordChemical Biologyen_US
dc.subject.keywordMolecular Dynamicsen_US
dc.subject.keywordQuorum-Sensingen_US
dc.subject.keywordReceptor-Ligand Interactionsen_US
dc.subject.keywordStructural Fingerprintingen_US
dc.titleExploration of Chemical Diversity in Intercellular Quorum Sensing Signalling Systems in Prokaryotesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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[article-cris] Kemian tekniikan korkeakoulu / CHEM