Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorHaddad Momeni, Majiden_US
dc.contributor.authorFredslund, Folmeren_US
dc.contributor.authorBissaro, Bastienen_US
dc.contributor.authorRaji, Olanrewajuen_US
dc.contributor.authorVuong, Thu V.en_US
dc.contributor.authorMeier, Sebastianen_US
dc.contributor.authorNielsen, Tine Sofieen_US
dc.contributor.authorLombard, Vincenten_US
dc.contributor.authorGuigliarelli, Brunoen_US
dc.contributor.authorBiaso, Frédéricen_US
dc.contributor.authorHaon, Mireilleen_US
dc.contributor.authorGrisel, Sachaen_US
dc.contributor.authorHenrissat, Bernarden_US
dc.contributor.authorWelner, Ditte Hededamen_US
dc.contributor.authorMaster, Emma R.en_US
dc.contributor.authorBerrin, Jean Guyen_US
dc.contributor.authorAbou Hachem, Maheren_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorProtein Technologyen
dc.contributor.organizationNovo Nordisk Foundationen_US
dc.contributor.organizationAix-Marseille Universitéen_US
dc.contributor.organizationUniversity of Torontoen_US
dc.contributor.organizationTechnical University of Denmarken_US
dc.contributor.organizationNovo Nordisk A/Sen_US
dc.date.accessioned2021-05-05T06:16:47Z
dc.date.available2021-05-05T06:16:47Z
dc.date.issued2021-04-09en_US
dc.descriptionFunding Information: This study was supported by a Novo Nordisk Foundation Post-doc grant within the Biotechnology Based Synthesis and Production Research Programme to MHM (NNF17OC0025642). F.F. and D.H.W. were funded by The Novo Nordisk Foundation Grant No. NNF10CC1016517. NMR spectra were acquired at the NMR center DTU supported by the Villum and Carlsberg Foundations. J.G.B., B.G. (Bruno G) and F.B. (Frédéric B) thank IM2B (Institut de Microbiologie, Bioénergies et Biotechnologie). The DSC instrument is funded by the Carlsberg Foundation Grant (2013-01-0112) to M.A.H. The authors are also grateful to the EPR facilities available at the French EPR network (IR CNRS 3443) and the Aix-Marseille University EPR center. Prof. Peter Westh and Dr. Jeppi Kari are acknowledged for discussion on the redox-activities of AA7. Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
dc.description.abstractOxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative enzymes remains limited. Here, a phylogenetic analysis of the auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals a striking abundance of AA7-genes in phytopathogenic fungi and Oomycetes. Expression of five fungal enzymes, including three from unexplored clades, expands the AA7-substrate range and unveils a cellooligosaccharide dehydrogenase activity, previously unknown within AA7. Sequence and structural analyses identify unique signatures distinguishing the strict dehydrogenase clade from canonical AA7 oxidases. The discovered dehydrogenase directly is able to transfer electrons to an AA9 lytic polysaccharide monooxygenase (LPMO) and fuel cellulose degradation by LPMOs without exogenous reductants. The expansion of redox-profiles and substrate range highlights the functional diversity within AA7 and sets the stage for harnessing AA7 dehydrogenases to fine-tune LPMO activity in biotechnological conversion of plant feedstocks.en
dc.description.versionPeer revieweden
dc.format.extent13
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationHaddad Momeni, M, Fredslund, F, Bissaro, B, Raji, O, Vuong, T V, Meier, S, Nielsen, T S, Lombard, V, Guigliarelli, B, Biaso, F, Haon, M, Grisel, S, Henrissat, B, Welner, D H, Master, E R, Berrin, J G & Abou Hachem, M 2021, ' Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs ', Nature Communications, vol. 12, no. 1, 2132 . https://doi.org/10.1038/s41467-021-22372-0en
dc.identifier.doi10.1038/s41467-021-22372-0en_US
dc.identifier.issn2041-1723
dc.identifier.otherPURE UUID: 059f01fe-4238-4004-af5d-8c33e26e5b09en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/059f01fe-4238-4004-af5d-8c33e26e5b09en_US
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dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/62347351/CHEM_Momeni_et_al_Discovery_of_fungal_2021_Nature_Communications.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/107194
dc.identifier.urnURN:NBN:fi:aalto-202105056458
dc.language.isoenen
dc.publisherNature Publishing Group
dc.relation.ispartofseriesNature Communicationsen
dc.relation.ispartofseriesVolume 12, issue 1en
dc.rightsopenAccessen
dc.titleDiscovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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[article-cris] Kemian tekniikan korkeakoulu / CHEM