Self-Coacervation of a Silk-Like Protein and Its Use As an Adhesive for Cellulosic Materials

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorMohammadi, Pezhmanen_US
dc.contributor.authorBeaune, Grégoryen_US
dc.contributor.authorStokke, Bjørn Torgeren_US
dc.contributor.authorTimonen, Jaakko V.I.en_US
dc.contributor.authorLinder, Markus B.en_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorBiomolecular Materialsen
dc.contributor.groupauthorActive Matteren
dc.contributor.organizationNorwegian University of Science and Technologyen_US
dc.date.accessioned2018-12-10T10:28:25Z
dc.date.available2018-12-10T10:28:25Z
dc.date.issued2018-09-18en_US
dc.description.abstractLiquid-liquid phase separation of biomacromolecules plays a critical role in many of their functions, both as cellular components and in structural assembly. Phase separation is also a key mechanism in the assembly of engineered recombinant proteins for the general aim to build new materials with unique structures and properties. Here the phase separation process of an engineered protein with a block-architecture was studied. As a central block, we used a modified spider silk sequence, predicted to be unstructured. In each terminus, folded globular blocks were used. We studied the kinetics and mechanisms of phase formation and analyzed the evolving structures and their viscoelastic properties. Individual droplets were studied with a micropipette technique, showing both how properties vary between individual drops and explaining overall bulk rheological properties. A very low surface energy allowed easy deformation of droplets and led to efficient infiltration into cellulosic fiber networks. Based on these findings, we demonstrated an efficient use of the phase-separated material as an adhesive for cellulose. We also conclude that the condensed state is metastable, showing an ensemble of properties in individual droplets and that an understanding of protein phase behavior will lead to developing a wider use of proteins as structural polymers.en
dc.description.versionPeer revieweden
dc.format.extent6
dc.format.extent1120-1125
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationMohammadi, P, Beaune, G, Stokke, B T, Timonen, J V I & Linder, M B 2018, ' Self-Coacervation of a Silk-Like Protein and Its Use As an Adhesive for Cellulosic Materials ', ACS Macro Letters, vol. 7, no. 9, pp. 1120-1125 . https://doi.org/10.1021/acsmacrolett.8b00527en
dc.identifier.doi10.1021/acsmacrolett.8b00527en_US
dc.identifier.issn2161-1653
dc.identifier.otherPURE UUID: b9ecf572-c340-42f0-8e4b-501d4ca2d6eeen_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/b9ecf572-c340-42f0-8e4b-501d4ca2d6eeen_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85053670851&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/29763870/acsmacrolett.8b00527.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/35239
dc.identifier.urnURN:NBN:fi:aalto-201812106254
dc.language.isoenen
dc.relation.ispartofseriesACS Macro Lettersen
dc.relation.ispartofseriesVolume 7, issue 9en
dc.rightsopenAccessen
dc.titleSelf-Coacervation of a Silk-Like Protein and Its Use As an Adhesive for Cellulosic Materialsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion

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