Reference nanoparticle measurements for biological measurements
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URL
Journal Title
Journal ISSN
Volume Title
Kemian tekniikan korkeakoulu |
Master's thesis
Authors
Date
2022-10-18
Department
Major/Subject
Functional Materials for Global Challenges
Mcode
CHEM3061
Degree programme
Master's Programme in Advanced Materials for Innovation and Sustainability
Language
en
Pages
49+8
Series
Abstract
As of now, there is no standard reference sample or method for the measurement of the Extracellular Vesicles (EVs), and existing reference samples exhibit a different refractive index than EV particles. METVES II project (EMPIR) strives to demonstrate a standard method for EV measurements, and this thesis work carried out in VTT MIKES is a part of the metrological size measurements traceability of EVs, which calibrates an Atomic Force Microscope and prepares nanoparticle standard reference samples for the calibration of FCM and NT A instruments for EV measurements. This work includes AFM calibration processes using reference nanoparticle samples, sample preparation of a new type of reference sample and running AFM measurements, AFM data analysis, and measurement uncertainty evaluation.Description
Supervisor
Koskinen, JariThesis advisor
Korpelainen, VirpiKeywords
nanoparticles, AFM, metrology, traceability, uncertainty, extracellular vesicles