Reference nanoparticle measurements for biological measurements

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Journal Title
Journal ISSN
Volume Title
Kemian tekniikan korkeakoulu | Master's thesis
Date
2022-10-18
Department
Major/Subject
Functional Materials for Global Challenges
Mcode
CHEM3061
Degree programme
Master's Programme in Advanced Materials for Innovation and Sustainability
Language
en
Pages
49+8
Series
Abstract
As of now, there is no standard reference sample or method for the measurement of the Extracellular Vesicles (EVs), and existing reference samples exhibit a different refractive index than EV particles. METVES II project (EMPIR) strives to demonstrate a standard method for EV measurements, and this thesis work carried out in VTT MIKES is a part of the metrological size measurements traceability of EVs, which calibrates an Atomic Force Microscope and prepares nanoparticle standard reference samples for the calibration of FCM and NT A instruments for EV measurements. This work includes AFM calibration processes using reference nanoparticle samples, sample preparation of a new type of reference sample and running AFM measurements, AFM data analysis, and measurement uncertainty evaluation.
Description
Supervisor
Koskinen, Jari
Thesis advisor
Korpelainen, Virpi
Keywords
nanoparticles, AFM, metrology, traceability, uncertainty, extracellular vesicles
Other note
Citation