Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorRomero-Mangado, Jaione
dc.contributor.authorNordlund, Dennis
dc.contributor.authorSoberon, Felipe
dc.contributor.authorDeane, Graham
dc.contributor.authorMaughan, Kevin
dc.contributor.authorSainio, Sami
dc.contributor.authorSingh, Gurusharan
dc.contributor.authorDaniels, Stephen
dc.contributor.authorSaunders, Ian T.
dc.contributor.authorLoftus, David
dc.contributor.authorMeyyappan, M.
dc.contributor.authorKoehne, Jessica
dc.contributor.authorGandhiraman, Ram P.
dc.contributor.departmentNASA Ames Research Center
dc.contributor.departmentStanford University
dc.contributor.departmentNovaerus, Inc.
dc.contributor.departmentDepartment of Electrical Engineering and Automation
dc.contributor.departmentDublin City University
dc.contributor.departmentUniversities Space Research Association
dc.date.accessioned2018-09-04T11:14:16Z
dc.date.available2018-09-04T11:14:16Z
dc.date.issued2016-03-01
dc.description.abstractThis study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reduction in amine functionalities and phosphate groups. Near edge x-ray absorption fine structure (NEXAFS) measurements confirm the presence of additional oxide bonds upon DBD treatment, suggesting oxidation of the outer layer of the cell wall. Electron microscopy images show that the bacteria undergo physical distortion to varying degrees, resulting in deformation of the bacterial structure. The electromagnetic field around the DBD coil causes severe damage to the cell structure, possibly resulting in leakage of vital cellular materials. The oxidation and chemical modification of the bacterial components are evident from the Fourier transform infrared spectroscopy and NEXAFS results. The bacterial reculture experiments confirm inactivation of airborne E. coli upon treating with DBD.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdf
dc.identifier.citationRomero-Mangado , J , Nordlund , D , Soberon , F , Deane , G , Maughan , K , Sainio , S , Singh , G , Daniels , S , Saunders , I T , Loftus , D , Meyyappan , M , Koehne , J & Gandhiraman , R P 2016 , ' Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge ' , Biointerphases , vol. 11 , no. 1 , 011009 . https://doi.org/10.1116/1.4941367en
dc.identifier.doi10.1116/1.4941367
dc.identifier.issn1934-8630
dc.identifier.otherPURE UUID: d7faf0ec-fdb8-4750-9a52-dcc521f29a69
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/d7faf0ec-fdb8-4750-9a52-dcc521f29a69
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=84958534696&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/27537623/1.4941367.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/33824
dc.identifier.urnURN:NBN:fi:aalto-201809044944
dc.language.isoenen
dc.relation.ispartofseriesBIOINTERPHASESen
dc.relation.ispartofseriesVolume 11, issue 1en
dc.rightsopenAccessen
dc.subject.keywordbacteria
dc.subject.keywordx-ray absorption
dc.subject.keywordfourier transforms
dc.titleMorphological and chemical changes of aerosolized E. coli treated with a dielectric barrier dischargeen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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