Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2020-09-15
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Language
en
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Materials Letters, Volume 275
Abstract
DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.Description
Keywords
Aptamer, Dissociation constant, DNA origami, Glycated hemoglobin, Surface plasmon resonance sensor
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Citation
Duanghathaipornsuk , S , Shen , B , Cameron , B D , Ijäs , H , Linko , V , Kostiainen , M & Kim , D-S 2020 , ' Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor ' , Materials Letters , vol. 275 , 128141 . https://doi.org/10.1016/j.matlet.2020.128141