Food-Grade Quercetin-Loaded Nanoemulsion Ameliorates Effects Associated with Parkinson’s Disease and Cancer: Studies Employing a Transgenic C. elegans Model and Human Cancer Cell Lines
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2022-07
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en
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14
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Antioxidants, Volume 11, issue 7, pp. 1-14
Abstract
A nanosized food-grade quercetin-loaded nanoemulsion (QNE) system comprising capmul MCM NF (oil) and cremophor RH 40 (surfactant) was developed using a high-speed homogenization technique. The developed QNE was studied for its significant neuroprotective (anti-Parkinsonism) and cytotoxicity (anticancer) effects against Caenorhabditis elegans (C. elegans) strains and human cancer cells, respectively. HR-TEM studies revealed that the QNE was spherical with a mean globule size of ~50 nm. Selected area electron diffraction (SAED) studies results demonstrated that QNE was amorphous. In vivo results show that QNE potentially reduced the α-Syn aggregation, increased mitochondrial and fat content, and improved the lifespan in transgenic C. elegans strain NL5901. QNE significantly downregulated the reactive oxygen species (ROS) levels in wild-type C. elegans strain N2. In vitro results of the MTT assay show that QNE significantly exhibited chemotherapeutic effects in all treated human cancer cells in an order of cytotoxicity: HeLa cells > A549 cells > MIA PaCa-2 cells, based on the IC50 values at 24 h. Conclusively, the QNE showed improved solubility, targetability, and neuroprotective effects against the PD-induced C. elegans model, and also cytotoxicity against human cancer cells and could be potentially used as an anti-Parkinson’s or anticancer agent.Description
Funding Information: The APC was funded by Kavindra Kesari, Aalto University, Finland. Publisher Copyright: © 2022 by the authors.
Keywords
Caenorhabditis elegans, chemotherapeutic effect, food-grade quercetin nanoemulsion, neuroprotective effect, Parkinson’s disease
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Das, S S, Sarkar, A, Chabattula, S C, Verma, P R P, Nazir, A, Gupta, P K, Ruokolainen, J, Kesari, K K & Singh, S K 2022, ' Food-Grade Quercetin-Loaded Nanoemulsion Ameliorates Effects Associated with Parkinson’s Disease and Cancer: Studies Employing a Transgenic C. elegans Model and Human Cancer Cell Lines ', Antioxidants, vol. 11, no. 7, 1378, pp. 1-14 . https://doi.org/10.3390/antiox11071378