Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media

dc.contributorAalto Universityen
dc.contributor.authorSipponen, Mika Henrikkien_US
dc.contributor.authorFarooq, Muhammaden_US
dc.contributor.authorKoivisto, Jarien_US
dc.contributor.authorPellis, Alessandroen_US
dc.contributor.authorSeitsonen, Janien_US
dc.contributor.authorÖsterberg, Monikaen_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorBioproduct Chemistryen
dc.contributor.organizationUniversity of Yorken_US
dc.description.abstractDehydration reactions proceed readily in water-filled biological cells. Development of biocatalysts that mimic such compartmentalized reactions has been cumbersome due to the lack of low-cost nanomaterials and associated technologies. Here we show that cationic lignin nanospheres function as activating anchors for hydrolases, and enable aqueous ester synthesis by forming spatially confined biocatalysts upon self-assembly and drying-driven aggregation in calcium alginate hydrogel. Spatially confined microbial cutinase and lipase retain 97% and 70% of their respective synthetic activities when the volume ratio of water to hexane increases from 1:1 to 9:1 in the reaction medium. The activity retention of industrially most frequently used acrylic resin-immobilized Candida antarctica lipase B is only 51% under similar test conditions. Overall, our findings enable fabrication of robust renewable biocatalysts for aqueous ester synthesis, and provide insight into the compartmentalization of diverse heterogeneous catalysts.en
dc.description.versionPeer revieweden
dc.identifier.citationSipponen, M H, Farooq, M, Koivisto, J, Pellis, A, Seitsonen, J & Österberg, M 2018, ' Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media ', Nature Communications, vol. 9, no. 1, 2300 .
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dc.relation.ispartofseriesNature Communicationsen
dc.relation.ispartofseriesVolume 9, issue 1en
dc.titleSpatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous mediaen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi