Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorSipponen, Mika Henrikkien_US
dc.contributor.authorFarooq, Muhammaden_US
dc.contributor.authorKoivisto, Jarien_US
dc.contributor.authorPellis, Alessandroen_US
dc.contributor.authorSeitsonen, Janien_US
dc.contributor.authorÖsterberg, Monikaen_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.departmentDepartment of Chemistry and Materials Scienceen
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorBioproduct Chemistryen
dc.contributor.organizationUniversity of Yorken_US
dc.date.accessioned2018-06-29T12:49:47Z
dc.date.available2018-06-29T12:49:47Z
dc.date.issued2018-12-01en_US
dc.description.abstractDehydration reactions proceed readily in water-filled biological cells. Development of biocatalysts that mimic such compartmentalized reactions has been cumbersome due to the lack of low-cost nanomaterials and associated technologies. Here we show that cationic lignin nanospheres function as activating anchors for hydrolases, and enable aqueous ester synthesis by forming spatially confined biocatalysts upon self-assembly and drying-driven aggregation in calcium alginate hydrogel. Spatially confined microbial cutinase and lipase retain 97% and 70% of their respective synthetic activities when the volume ratio of water to hexane increases from 1:1 to 9:1 in the reaction medium. The activity retention of industrially most frequently used acrylic resin-immobilized Candida antarctica lipase B is only 51% under similar test conditions. Overall, our findings enable fabrication of robust renewable biocatalysts for aqueous ester synthesis, and provide insight into the compartmentalization of diverse heterogeneous catalysts.en
dc.description.versionPeer revieweden
dc.format.extent7
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationSipponen, M H, Farooq, M, Koivisto, J, Pellis, A, Seitsonen, J & Österberg, M 2018, ' Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media ', Nature Communications, vol. 9, no. 1, 2300 . https://doi.org/10.1038/s41467-018-04715-6en
dc.identifier.doi10.1038/s41467-018-04715-6en_US
dc.identifier.issn2041-1723
dc.identifier.otherPURE UUID: bfde67ea-4fba-4940-a6ff-22e1a572fdb9en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/bfde67ea-4fba-4940-a6ff-22e1a572fdb9en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85048370996&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/26025556/Sipponen_et_al_Spatially_confined_2018_Nature_Communications.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/32614
dc.identifier.urnURN:NBN:fi:aalto-201806294024
dc.language.isoenen
dc.relation.ispartofseriesNature Communicationsen
dc.relation.ispartofseriesVolume 9, issue 1en
dc.rightsopenAccessen
dc.titleSpatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous mediaen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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