Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2018-12-01
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Language
en
Pages
7
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Nature Communications, Volume 9, issue 1
Abstract
Dehydration reactions proceed readily in water-filled biological cells. Development of biocatalysts that mimic such compartmentalized reactions has been cumbersome due to the lack of low-cost nanomaterials and associated technologies. Here we show that cationic lignin nanospheres function as activating anchors for hydrolases, and enable aqueous ester synthesis by forming spatially confined biocatalysts upon self-assembly and drying-driven aggregation in calcium alginate hydrogel. Spatially confined microbial cutinase and lipase retain 97% and 70% of their respective synthetic activities when the volume ratio of water to hexane increases from 1:1 to 9:1 in the reaction medium. The activity retention of industrially most frequently used acrylic resin-immobilized Candida antarctica lipase B is only 51% under similar test conditions. Overall, our findings enable fabrication of robust renewable biocatalysts for aqueous ester synthesis, and provide insight into the compartmentalization of diverse heterogeneous catalysts.
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Sipponen, M H, Farooq, M, Koivisto, J, Pellis, A, Seitsonen, J & Österberg, M 2018, ' Spatially confined lignin nanospheres for biocatalytic ester synthesis in aqueous media ', Nature Communications, vol. 9, no. 1, 2300 . https://doi.org/10.1038/s41467-018-04715-6