Acetone-butanol-ethanol (ABE) fermentation by clostridium acetobutylicum using xylose recovered from birch wood hydrolysis

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Kemian tekniikan korkeakoulu | Master's thesis
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Date

2018-10-02

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Major/Subject

Chemical and Process Engineering

Mcode

CHEM3043

Degree programme

Master's Programme in Chemical, Biochemical and Materials Engineering

Language

en

Pages

30

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Abstract

The focus of this study is to find the efficiency of xylose and birch wood hydrolysate in Acetone-butanol-ethanol (ABE) fermentation. There is a need to find an effective raw material for the commercial production of butanol which was the reason for this work to be carried out. The literature studies include the background and biochemistry of the process, different types of microorganisms used for this process, pretreatment methods for hydrolysate and detoxification of hydrolysate. The experimental part of this thesis is about finding the effect of glucose and xylose, effect of electron carriers, effect of different concentration of the potent electron carrier and the efficiency of birch wood hydrolysate in ABE fermentation. The results obtained are compared with the results from previous works of different researchers and the discussion are done based on it. The results showed that 60:40 (xylose:glucose) is the best ratio as it yielded 7.99 g/L of overall solvent concentration and also lower residual acid of about 2.68 g/L. Methylene blue was found to give higher solvent concentration of about 9.48 g/L when compared to other electron carriers. The concentration of 0.1 mM methylene blue produced higher solvent concentration and A:B ratio which was about 9.47 g/L and 2.82:1. From this work, it was found that birch wood hydrolysate can be very effective for the ABE fermentation if proper pretreatment and detoxification methods are carried out.

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Supervisor

Banksr, Sandip

Thesis advisor

Chandgude, Vijaya

Keywords

ABE fermentation, birch wood hydrolysate, xylose, electron carrier

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https://urn.fi/URN:NBN:fi:aalto-201810055273

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[dipl] Kemian tekniikan korkeakoulu / CHEM