Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Authors
Yu, Tianyi
Anbarasan, Sasikala
Wang, Yawei
Telli, Kübra
Aslan, Aşkın Sevinç
Su, Zhengding
Zhou, Yin
Zhang, Li
Iivonen, Piia
Havukainen, Sami
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en
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10
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Extremophiles, Volume 20, issue 4, pp. 515–524
Abstract
The gene of Thermotoga maritima GH10 xylanase (TmXYN10B) was synthesised to study the extreme limits of this hyperthermostable enzyme at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids (ILs). TmXYN10B expressed from Pichia pastoris showed maximal activity at 100 °C and retained 92 % of maximal activity at 105 °C in a 30-min assay. Although the temperature optimum of activity was lowered by 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), TmXYN10B retained partial activity in 15–35 % hydrophilic ILs, even at 75–90 °C. TmXYN10B retained over 80 % of its activity at 90 °C in 15 % [EMIM]OAc and 15–25 % 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) during 22-h reactions. [EMIM]OAc may rigidify the enzyme and lower Vmax. However, only minor changes in kinetic parameter Km showed that competitive inhibition by [EMIM]OAc of TmXYN10B is minimal. In conclusion, when extended enzymatic reactions under extreme conditions are required, TmXYN10B shows extraordinary potential.Description
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Yu, T, Anbarasan, S, Wang, Y, Telli, K, Aslan, A S, Su, Z, Zhou, Y, Zhang, L, Iivonen, P, Havukainen, S, Mentunen, T, Hummel, M, Sixta, H, Binay, B, Turunen, O & Xiong, H 2016, 'Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids', Extremophiles, vol. 20, no. 4, pp. 515–524. https://doi.org/10.1007/s00792-016-0841-y