Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms
Loading...
Access rights
openAccess
publishedVersion
URL
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Date
Department
Major/Subject
Mcode
Degree programme
Language
en
Pages
6
Series
Proceedings of the National Academy of Sciences of the United States of America, Volume 113, issue 52, pp. 15066-15071
Abstract
In a screen for unexplained mutation events we identified a previously unrecognized mechanism generating clustered DNA polymorphisms such as microindels and cumulative SNPs. The mechanism, short-patch double illegitimate recombination (SPDIR), facilitates short single-stranded DNA molecules to invade and replace genomic DNA through two joint illegitimate recombination events. SPDIR is controlled by key components of the cellular genome maintenance machinery in the gram-negative bacterium Acinetobacter baylyi. The source DNA is primarily intragenomic but can also be acquired through horizontal gene transfer. The DNA replacements are nonreciprocal and locus independent. Bioinformatic approaches reveal occurrence of SPDIR events in the gram-positive human pathogen Streptococcus pneumoniae and in the human genome.Description
Keywords
Other note
Citation
Harms, K, Lunnan, A, Hülter, N, Mourier, T, Vinner, L, Andam, C P, Marttinen, P, Fridholm, H, Hansen, A J, Hanage, W P, Nielsen, K M, Willerslev, E & Johnsen, P J 2016, 'Substitutions of short heterologous DNA segments of intragenomic or extragenomic origins produce clustered genomic polymorphisms', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 52, pp. 15066-15071. https://doi.org/10.1073/pnas.1615819114