Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorLahdentausta, Laura
dc.contributor.authorKivimäki, Anne
dc.contributor.authorOksanen, Lotta
dc.contributor.authorTallgren, Marika
dc.contributor.authorOksanen, Sampo
dc.contributor.authorSanmark, Enni
dc.contributor.authorSalminen, Aino
dc.contributor.authorGeneid, Ahmed
dc.contributor.authorSairanen, Mikko
dc.contributor.authorPaju, Susanna
dc.contributor.authorSaksela, Kalle
dc.contributor.authorPussinen, Pirkko
dc.contributor.authorPietiäinen, Milla
dc.contributor.departmentUniversity of Helsinki
dc.contributor.departmentPerkinElmer Finland Oy
dc.contributor.departmentAalto University School of Business
dc.contributor.departmentDepartment of Accountingen
dc.date.accessioned2023-02-08T07:37:08Z
dc.date.available2023-02-08T07:37:08Z
dc.date.issued2022-08
dc.descriptionFunding Information: Open Access funding provided by University of Helsinki. The study has been supported by The Finnish Dental Society Apollonia (grant for MP and LL), the Helsinki and Uusimaa Hospital District (HUS, grant for LL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The data of this study has not been presented elsewhere. Publisher Copyright: © 2022, The Author(s).
dc.description.abstractWe examined the usefulness of dried spot blood and saliva samples in SARS-CoV-2 antibody analyses. We analyzed 1231 self-collected dried spot blood and saliva samples from healthcare workers. Participants filled in a questionnaire on their COVID-19 exposures, infections, and vaccinations. Anti-SARS-CoV-2 IgG, IgA, and IgM levels were determined from both samples using the GSP/DELFIA method. The level of exposure was the strongest determinant of all blood antibody classes and saliva IgG, increasing as follows: (1) no exposure (healthy, non-vaccinated), (2) exposed, (3) former COVID-19 infection, (4) one vaccination, (5) two vaccinations, and (6) vaccination and former infection. While the blood IgG assay had a 99.5% sensitivity and 75.3% specificity to distinguish participants with two vaccinations from all other types of exposure, the corresponding percentages for saliva IgG were 85.3% and 65.7%. Both blood and saliva IgG-seropositivity proportions followed similar trends to the exposures reported in the questionnaires. Self-collected dry blood and saliva spot samples combined with the GSP/DELFIA technique comprise a valuable tool to investigate an individual’s immune response to SARS-CoV-2 exposure or vaccination. Saliva IgG has high potential to monitor vaccination response wane, since the sample is non-invasive and easy to collect.en
dc.description.versionPeer revieweden
dc.format.extent11
dc.format.extent173-183
dc.format.mimetypeapplication/pdf
dc.identifier.citationLahdentausta , L , Kivimäki , A , Oksanen , L , Tallgren , M , Oksanen , S , Sanmark , E , Salminen , A , Geneid , A , Sairanen , M , Paju , S , Saksela , K , Pussinen , P & Pietiäinen , M 2022 , ' Blood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samples ' , Medical Microbiology and Immunology , vol. 211 , no. 4 , pp. 173-183 . https://doi.org/10.1007/s00430-022-00740-xen
dc.identifier.doi10.1007/s00430-022-00740-x
dc.identifier.issn0300-8584
dc.identifier.issn1432-1831
dc.identifier.otherPURE UUID: beb3b210-6c89-4a09-aa61-0ea93ebfc6db
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/beb3b210-6c89-4a09-aa61-0ea93ebfc6db
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85131730304&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/99646156/s00430_022_00740_x.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/119699
dc.identifier.urnURN:NBN:fi:aalto-202302082049
dc.language.isoenen
dc.publisherSPRINGER
dc.relation.ispartofseriesMedical Microbiology and Immunologyen
dc.relation.ispartofseriesVolume 211, issue 4en
dc.rightsopenAccessen
dc.subject.keywordAntibody
dc.subject.keywordBlood
dc.subject.keywordCOVID-19
dc.subject.keywordDried spot sample
dc.subject.keywordExposure
dc.subject.keywordSaliva
dc.subject.keywordSARS-CoV-2
dc.subject.keywordVaccination
dc.titleBlood and saliva SARS-CoV-2 antibody levels in self-collected dried spot samplesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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