Colorimetric detection of acetyl xylan esterase activities
| dc.contributor | Aalto-yliopisto | fi |
| dc.contributor | Aalto University | en |
| dc.contributor.author | Mai-Gisondi, Galina | en_US |
| dc.contributor.author | Master, Emma R. | en_US |
| dc.contributor.department | Department of Bioproducts and Biosystems | en |
| dc.contributor.editor | Abbott, D. Wade | en_US |
| dc.contributor.editor | Lammerts van Bueren, Alicia | en_US |
| dc.contributor.groupauthor | Molecular biotechnology | en |
| dc.date.accessioned | 2019-01-14T09:24:19Z | |
| dc.date.available | 2019-01-14T09:24:19Z | |
| dc.date.issued | 2017 | en_US |
| dc.description | | openaire: EC/H2020/33947/EU//BHIVE | |
| dc.description.abstract | Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-d-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted. | en |
| dc.description.version | Peer reviewed | en |
| dc.format.extent | 13 | |
| dc.identifier.citation | Mai-Gisondi, G & Master, E R 2017, Colorimetric detection of acetyl xylan esterase activities. in D W Abbott & A Lammerts van Bueren (eds), Protein-Carbohydrate Interactions : Methods and Protocols. Methods in Molecular Biology, vol. 1588, Springer, pp. 45-57. https://doi.org/10.1007/978-1-4939-6899-2_5 | en |
| dc.identifier.doi | 10.1007/978-1-4939-6899-2_5 | en_US |
| dc.identifier.isbn | 978-1-4939-6898-5 | |
| dc.identifier.isbn | 978-1-4939-6899-2 | |
| dc.identifier.issn | 10643745 | |
| dc.identifier.other | PURE UUID: cbb986cf-e365-475f-9237-a99e548d74c8 | en_US |
| dc.identifier.other | PURE ITEMURL: https://research.aalto.fi/en/publications/cbb986cf-e365-475f-9237-a99e548d74c8 | en_US |
| dc.identifier.uri | https://aaltodoc.aalto.fi/handle/123456789/36020 | |
| dc.identifier.urn | URN:NBN:fi:aalto-201901141203 | |
| dc.language.iso | en | en |
| dc.relation | info:eu-repo/grantAgreement/EC/H2020/33947/EU//BHIVE | en_US |
| dc.relation.ispartof | Protein-Carbohydrate Interactions: Methods and Protocols | en |
| dc.relation.ispartof | pp. 45-57 | en |
| dc.relation.ispartofseries | Methods in Molecular Biology ; Volume 1588 | en |
| dc.rights | restrictedAccess | en |
| dc.subject.keyword | 4-Methylumbelliferyl acetate | en_US |
| dc.subject.keyword | Acetyl xylan esterase | en_US |
| dc.subject.keyword | Acetylated xylooligosaccharides | en_US |
| dc.subject.keyword | Colorimetric assays | en_US |
| dc.subject.keyword | pNP-acetate | en_US |
| dc.subject.keyword | α-Naphthyl acetate | en_US |
| dc.subject.keyword | β-Xylosidase-coupled assay | en_US |
| dc.title | Colorimetric detection of acetyl xylan esterase activities | en |
| dc.type | A3 Kirjan tai muun kokoomateoksen osa | fi |