Colorimetric detection of acetyl xylan esterase activities

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A3 Kirjan tai muun kokoomateoksen osa
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Date
2017
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Language
en
Pages
13
45-57
Series
Protein-Carbohydrate Interactions, Methods in Molecular Biology, Volume 1588
Abstract
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-d-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted.
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| openaire: EC/H2020/33947/EU//BHIVE
Keywords
4-Methylumbelliferyl acetate, Acetyl xylan esterase, Acetylated xylooligosaccharides, Colorimetric assays, pNP-acetate, α-Naphthyl acetate, β-Xylosidase-coupled assay
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Citation
Mai-Gisondi , G & Master , E R 2017 , Colorimetric detection of acetyl xylan esterase activities . in D W Abbott & A Lammerts van Bueren (eds) , Protein-Carbohydrate Interactions : Methods and Protocols . Methods in Molecular Biology , vol. 1588 , Springer , pp. 45-57 . https://doi.org/10.1007/978-1-4939-6899-2_5