Colorimetric detection of acetyl xylan esterase activities

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A3 Kirjan tai muun kokoomateoksen osa
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Date

2017

Department

Department of Bioproducts and Biosystems

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Mcode

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Language

en

Pages

13

Series

Methods in Molecular Biology ; Volume 1588

Abstract

Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-d-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted.

Description

| openaire: EC/H2020/33947/EU//BHIVE

Keywords

4-Methylumbelliferyl acetate, Acetyl xylan esterase, Acetylated xylooligosaccharides, Colorimetric assays, pNP-acetate, α-Naphthyl acetate, β-Xylosidase-coupled assay

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DOI

10.1007/978-1-4939-6899-2_5

Citation

Mai-Gisondi, G & Master, E R 2017, Colorimetric detection of acetyl xylan esterase activities. in D W Abbott & A Lammerts van Bueren (eds), Protein-Carbohydrate Interactions : Methods and Protocols. Methods in Molecular Biology, vol. 1588, Springer, pp. 45-57. https://doi.org/10.1007/978-1-4939-6899-2_5

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https://urn.fi/URN:NBN:fi:aalto-201901141203

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[article-cris] Kemian tekniikan korkeakoulu / CHEM