Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-01
Major/Subject
Mcode
Degree programme
Language
en
Pages
103-110
Series
Virology, Volume 578
Abstract
Potato virus A (PVA) is a plant-infecting RNA virus that produces flexible particles with a high aspect ratio. PVA has been investigated extensively for its infection biology, however, its potential to serve as a nanopatterning platform remains unexplored. Here, we study the liquid crystal and interfacial self-assembly behavior of PVA particles. Furthermore, we generate nanopatterned surfaces using self-assembled PVA particles through three different coating techniques: drop-casting, drop-top deposition and flow-coating. The liquid crystal phase of PVA solution visualized by polarized optical microscopy revealed a chiral nematic phase in water, while in pH 8 buffer it produced a nematic phase. This allowed us to produce thin films with either randomly or anisotropically oriented cylindrical nanopatterns using drop-top and flow-coating methods. Overall, this study explores the self-assembly process of PVA in different conditions, establishing a starting point for PVA self-assembly research and contributing a virus-assisted fabrication technique for nanopatterned surfaces.
Description
| openaire: EC/H2020/949648/EU//ModelCom The research in Multifunctional Material Design lab (Aalto University) was funded by The Ella Georg Ehrnrooth Foundation, the European Research Council (H2020) StG “Autonomously adapting and communicating modular textiles” No. 949648 ModelCom, Academy of Finland SUPER-WEAR project (decision number: 322214), ProCrystal (decision 680210), Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Research in the Mäkinen lab (University of Helsinki) is supported by Academy of Finland (decision 332950). Further, we acknowledge OtaNano Nanomicroscopy Center at Aalto University for their facilities and expertise that were crucial for the SAXS and TEM studies carried out in this work. We also Acknowledge HiLIFE Biocomplex unit at the University of Helsinki, a member of Instruct-ERIC Centre Finland, FINStruct, and Biocenter Finland for their facilities and expertise that were crucial for the purification of PVA VNPs.
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Citation
De , S , Nguyen , H M , Liljeström , V , Mäkinen , K , Kostiainen , M & Vapaavuori , J 2023 , ' Potato virus A particles – A versatile material for self-assembled nanopatterned surfaces ' , Virology , vol. 578 , pp. 103-110 . https://doi.org/10.1016/j.virol.2022.11.010