PLA2 Interfacial Activation on Lipid Interfaces Promoting Fibril Formation

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School of Science | Doctoral thesis (article-based) | Defence date: 2013-11-01
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Date
2013
Major/Subject
Mcode
Degree programme
Language
en
Pages
66 + app. 44
Series
Aalto University publication series DOCTORAL DISSERTATIONS, 164/2013
Abstract
Phospholipase A2 is a widely studied protein found ubiquitously in nature. The protein interacts with the lipid membrane cleaving the phospholipid at the sn-2 position. The interfacial activation mechanism is characterized by a low activity on monomeric substrates but a greatly enhanced activity on aggregated substrates. Several of our papers have elucidated the complex interfacial activation mechanism of this protein from different angles in two very diverse scientific frames: from the view of the lipid and the view of the protein. In this thesis the interfacial activation mechanism of PLA2 is understood to behave in a temporal sequence of events first by binding, dimer formation, oligomer formation and finally by the formation of amyloid-like fibrils.
Description
Supervising professor
Kinnunen, Paavo K. J., Prof., Aalto University, Department of Biomedical Engineering and Computational Science, Finland
Thesis advisor
Kinnunen, Paavo K. J., Prof., Aalto University, Department of Biomedical Engineering and Computational Science, Finland
Keywords
liposome, PLA2, peptide, phospholipid, amyloid, protein-lipid interaction, dimer, temporin B, heat-shock protein, oxidized phospholipids
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Parts
  • [Publication 1]: Code C, Domanov Y, Jutila A, Kinnunen PK. Amyloid-type fiber formation in control of enzyme action: interfacial activation of phospholipase A2. Biophys J. 2008 Jul;95(1):215-24.
  • [Publication 2]: Code C, Domanov YA, Killian JA, Kinnunen PK. Activation of phospholipase A(2) by temporin B: Formation of antimicrobial peptide-enzyme amyloid-type cofibrils. Biochim Biophys Acta. 2009 May;1788(5):1064-72.
  • [Publication 3]: Code C, Mahalka AK, Bry K, Kinnunen PK. Activation of phospholipase A2 by 1-palmitoyl-2-(9'-oxo-nonanoyl)-sn-glycero-3-phosphocholine in vitro. Biochim Biophys Acta. 2010 Aug;1798(8):1593-600.
  • [Publication 4]: Mahalka AK, Code C, Rezaijahromi B, Kirkegaard T, Jäättelä M, Kinnunen PK. Activation of phospholipase A2 by HSP70 in vitro. Biochim Biophys Acta. 2011 Oct;1808 (10): 2569–72.
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