Enhanced polymer capture speed and extended translocation time in pressure-solvation traps

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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2018-06-11

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en

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1-13

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Physical Review E, Volume 97, issue 6

Abstract

The efficiency of nanopore-based biosequencing techniques requires fast anionic polymer capture by like-charged pores followed by a prolonged translocation process. We show that this condition can be achieved by setting a pressure-solvation trap. Polyvalent cation addition to the KCl solution triggers the like-charge polymer-pore attraction. The attraction speeds-up the pressure-driven polymer capture but also traps the molecule at the pore exit, reducing the polymer capture time and extending the polymer escape time by several orders of magnitude. By direct comparison with translocation experiments [D. P. Hoogerheide, ACS Nano 8, 7384 (2014)1936-085110.1021/nn5025829], we characterize as well the electrohydrodynamics of polymers transport in pressure-voltage traps. We derive scaling laws that can accurately reproduce the pressure dependence of the experimentally measured polymer translocation velocity and time. We also find that during polymer capture, the electrostatic barrier on the translocating molecule slows down the liquid flow. This prediction identifies the streaming current measurement as a potential way to probe electrostatic polymer-pore interactions.

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Buyukdagli, S 2018, ' Enhanced polymer capture speed and extended translocation time in pressure-solvation traps ', Physical Review E, vol. 97, no. 6, 062406, pp. 1-13 . https://doi.org/10.1103/PhysRevE.97.062406