Correct Identification of the Core-Shell Structure of Cell Membrane-Coated Polymeric Nanoparticles
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2022-12-06
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en
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Chemistry - A European Journal, Volume 28, issue 68
Abstract
Transmission electron microscopy (TEM) observations of negatively stained cell membrane (CM)-coated polymeric nanoparticles (NPs) reveal a characteristic core-shell structure. However, negative staining agents can create artifacts that complicate the determination of the actual NP structure. Herein, it is demonstrated with various bare polymeric core NPs, such as poly(lactic-co-glycolic acid) (PLGA), poly(ethylene glycol) methyl ether-block-PLGA, and poly(caprolactone), that certain observed core-shell structures are actually artifacts caused by the staining process. To address this issue, fluorescence quenching was applied to quantify the proportion of fully coated NPs and statistical TEM analysis was used to identify and differentiate whether the observed core-shell structures of CM-coated PLGA (CM−PLGA) NPs are due to artifacts or to the CM coating. Integrated shells in TEM images of negatively stained CM−PLGA NPs are identified as artifacts. The present results challenge current understanding of the structure of CM-coated polymeric NPs and encourage researchers to use the proposed characterization approach to avoid misinterpretations.Description
Funding Information: The authors thank Jari Leskinen and Virpi Miettinen at SIB Labs of the University of Eastern Finland for technical support in TEM imaging. This work was supported by the Academy of Finland (projects 314412), and the Ganjiang New Area: Program for High‐Level Talents Introduction. Publisher Copyright: © 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH.
Keywords
artifacts, cell membrane coating, core-shell structure, negative staining, polymeric nanoparticles
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Liu, L, Yu, W, Seitsonen, J, Xu, W & Lehto, V P 2022, ' Correct Identification of the Core-Shell Structure of Cell Membrane-Coated Polymeric Nanoparticles ', Chemistry: A European Journal, vol. 28, no. 68, e202200947 . https://doi.org/10.1002/chem.202200947