Exosome isolation and characterization for advanced diagnostic and therapeutic applications
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2025-04
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en
Pages
27
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Materials Today Bio, Volume 31, pp. 1-27
Abstract
Advancements in exosome isolation technologies are pivotal for transforming personalized medicine and enhancing clinical diagnostics. Exosomes, small extracellular vesicles with diameters ranging between 30 and 150 nm, are secreted into bodily fluids by a variety of cells and play essential roles in intercellular communication. These vesicles facilitate the transfer of nucleic acids, lipids, and proteins, affecting a wide range of biological and pathological processes. Given their importance in disease diagnostics, therapy, and as biomarkers, there has been a surge in developing methods to isolate them from fluids such as urine, saliva, blood, and cerebrospinal fluid. While traditional isolation techniques like ultracentrifugation and polymer-based precipitation have been foundational, recent technological advances have introduced more precise methods like microfluidics and immunoaffinity capture. These newer methods enable high-throughput and specific exosome isolation by targeting surface markers, thus enhancing purity. However, challenges such as balancing purity with yield and the lack of standardized protocols across different laboratories persist, impacting the consistency of findings. By integrating advanced isolation techniques and discussing their implications in diagnostics and therapy, this review aims to catalyze further research and adoption of exosome-based technologies in medicine, marking a significant stride towards tailored healthcare solutions.Description
Publisher Copyright: © 2025
Keywords
Exosome isolation, Exosome therapeutics, Immunoaffinity capture, Microfluidics, Ultracentrifugation
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Citation
Mukerjee, N, Bhattacharya, A, Maitra, S, Kaur, M, Ganesan, S, Mishra, S, Ashraf, A, Rizwan, M, Kesari, K K, Tabish, T A & Thorat, N D 2025, ' Exosome isolation and characterization for advanced diagnostic and therapeutic applications ', Materials Today Bio, vol. 31, 101613, pp. 1-27 . https://doi.org/10.1016/j.mtbio.2025.101613