Impact of ball milling on maize (Zea mays L.) stem structural components and on enzymatic hydrolysis of carbohydrates

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorSipponen, Mika Henrikki
dc.contributor.authorLaakso, Simo
dc.contributor.authorBaumberger, Stéphanie
dc.contributor.departmentDepartment of Biotechnology and Chemical Technologyen
dc.contributor.departmentBiotekniikan ja kemian tekniikan laitosfi
dc.contributor.labApplied Biochemistry Research Groupen
dc.contributor.labBiokemian tutkimusryhmäfi
dc.contributor.schoolKemian tekniikan korkeakoulufi
dc.contributor.schoolSchool of Chemical Technologyen
dc.date.accessioned2014-08-21T08:32:11Z
dc.date.available2014-08-21T08:32:11Z
dc.date.issued2014
dc.description.abstractThe impact of ball milling on structural components of maize (Zea mays L.) stem was investigated in relation to enzymatic hydrolysis of carbohydrates. Ball milling of extractive-free maize stem material was carried out with different milling times up to 12 h. Carbohydrate conversion from ball-milled maize stem material with cellulolytic preparation Onozuka R-10 increased to 79% with increasing ball milling time up to 4 h, but did not thereafter increase further. Ball milling caused drastic depolymerization of hemicelluloses as revealed by increasing amounts of monosaccharides released in absence of enzymes. Infrared spectroscopy showed that cellulose crystallinity decreased within the first 6 h milling thereafter reaching a plateau. Thioacidolysis of solid residue fractions obtained after treatment of ball-milled samples with Onozuka R-10 suggested that no extensive degradation of lignin occurred during the milling. The effect of lignin structure on enzymatic hydrolysis of associated carbohydrates was further studied independently of cellulose crystallinity. Two lignin-carbohydrate fractions comprising hemicellulose and lignin with either high or low amount of aryl ether linkages were used as model materials. Lignins abundant in aryl ether linkages appeared more detrimental than condensed lignins to enzymatic hydrolysis of associated carbohydrates.en
dc.format.extent130-136
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSipponen, Mika Henrikki & Laakso, Simo & Baumberger, Stéphanie. 2014. Impact of ball milling on maize (Zea mays L.) stem structural components and on enzymatic hydrolysis of carbohydrates. Industrial Crops and Products. Volume 61. P. 130-136. ISSN 0926-6690 (printed). DOI: 10.1016/j.indcrop.2014.06.052.en
dc.identifier.doi10.1016/j.indcrop.2014.06.052
dc.identifier.issn0926-6690 (printed)
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/13825
dc.identifier.urnURN:NBN:fi:aalto-201408212476
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.ispartofseriesIndustrial Crops and Productsen
dc.relation.ispartofseriesVolume 61
dc.rights.holderElsevier BV
dc.subject.keywordBall millingen
dc.subject.keywordmaize stemen
dc.subject.keywordstalken
dc.subject.keywordlignocelluloseen
dc.subject.keywordenzymaticen
dc.subject.keywordhydrolysisen
dc.subject.keywordligninen
dc.subject.keywordstructureen
dc.subject.otherBiotechnologyen
dc.titleImpact of ball milling on maize (Zea mays L.) stem structural components and on enzymatic hydrolysis of carbohydratesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.dcmitypetexten
dc.type.versionPre-printen
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