Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorSalo, Johanna M.en_US
dc.contributor.authorKedves, Orsolyaen_US
dc.contributor.authorMikkola, Raimoen_US
dc.contributor.authorKredics, Lászlóen_US
dc.contributor.authorAndersson, Maria A.en_US
dc.contributor.authorKurnitski, Jareken_US
dc.contributor.authorSalonen, Heidien_US
dc.contributor.departmentDepartment of Civil Engineeringen
dc.contributor.groupauthorStructures – Structural Engineering, Mechanics and Computationen
dc.contributor.organizationUniversity of Szegeden_US
dc.date.accessioned2020-08-06T12:18:03Z
dc.date.available2020-08-06T12:18:03Z
dc.date.issued2020-07-08en_US
dc.description.abstractThe diversity of Chaetomium-like isolates in buildings in Finland is poorly documented. This paper describes a set of methods for rapid diversity tracking of 42 indoor Chaetomium-like isolates. These isolates were categorized based on their fluorescence emission, ascomatal hair morphology, responses in three bioassays and resistance/sensitivity to the wetting agent Genapol X-080. Thirty-nine toxigenic isolates were identified [Ch. globosum (n = 35), Ch. cochliodes (n = 2) and Ch. rectangulare (n = 2)]. These isolates were identified down to the species level by tef1α gene sequencing. The major toxic substances in the ethanol extracts of the Ch. globosum and Ch. cochliodes strains were chaetoglobosin, chaetoviridin A and C, chaetomugilin D and chaetomin, identified based on HPLC-UV and mass spectrometry data (MS and MS/MS). Ethanol extracts from pure Ch. globosum cultures exhibited a toxicological profile in the boar sperm motility inhibition assay (BSMI), sperm membrane integrity damage assay (SMID) and inhibition of cell proliferation (ICP) assay, similar to that exhibited by pure chaetoglobosin A. Overall, differences in fluorescence, morphology, toxicity profile, mycotoxin production and sensitivity to chemicals were consistent with those in tef1α sequencing results for species identification. The results indicate the presence of Ch. cochliodes and Ch. rectangulare in Finnish buildings, representing a new finding.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationSalo, J M, Kedves, O, Mikkola, R, Kredics, L, Andersson, M A, Kurnitski, J & Salonen, H 2020, ' Detection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finland ', Toxins, vol. 12, no. 7, 443 . https://doi.org/10.3390/toxins12070443en
dc.identifier.doi10.3390/toxins12070443en_US
dc.identifier.issn2072-6651
dc.identifier.otherPURE UUID: eaaa4dae-7cc0-49d7-a8eb-686441172b7een_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/eaaa4dae-7cc0-49d7-a8eb-686441172b7een_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85088205376&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/44632843/toxins_12_00443_v3.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/45605
dc.identifier.urnURN:NBN:fi:aalto-202008064564
dc.language.isoenen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.ispartofseriesToxinsen
dc.relation.ispartofseriesVolume 12, issue 7en
dc.rightsopenAccessen
dc.subject.keywordchaetoglobosinen_US
dc.subject.keywordchaetominen_US
dc.subject.keywordChaetomium cochliodesen_US
dc.subject.keywordChaetomium globosumen_US
dc.subject.keywordChaetomium rectangulareen_US
dc.subject.keywordchaetomugilin Den_US
dc.subject.keywordchaetoviridin A and Cen_US
dc.subject.keywordfluorescenceen_US
dc.subject.keywordindoor molden_US
dc.subject.keywordmycotoxinen_US
dc.titleDetection of Chaetomium globosum, Ch. cochliodes and Ch. rectangulare during the Diversity Tracking of Mycotoxin-Producing Chaetomium-Like Isolates Obtained in Buildings in Finlanden
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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