Selective Antibacterial Activity and Lipid Membrane Interactions of Arginine-Rich Amphiphilic Peptides

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorEdwards-Gayle, Charlotte J.C.en_US
dc.contributor.authorBarrett, Glynen_US
dc.contributor.authorRoy, Shyamalien_US
dc.contributor.authorCastelletto, Valeriaen_US
dc.contributor.authorSeitsonen, Janien_US
dc.contributor.authorRuokolainen, Janneen_US
dc.contributor.authorHamley, Ian W.en_US
dc.contributor.departmentDepartment of Applied Physicsen
dc.contributor.groupauthorMolecular Materialsen
dc.contributor.organizationUniversity of Readingen_US
dc.date.accessioned2020-11-30T08:20:55Z
dc.date.available2020-11-30T08:20:55Z
dc.date.issued2020-02-17en_US
dc.description.abstractThe self-assembly behavior and antimicrobial activity of two designed amphiphilic peptides, R3F3 and R4F4, containing short hydrophobic phenylalanine (F) and cationic arginine (R) sequences, are investigated. The conformation of the peptides was examined using circular dichroism and FTIR spectroscopy, which show that they have a disordered secondary structure. Concentration-dependent fluorescence assays show the presence of a critical aggregation concentration (cac) for each peptide. Above the cac, small-angle X-ray scattering (SAXS) and transmission electron microscopy (TEM) reveal a population of twisted tapes for R3F3 and nanosheets for R4F4. The interaction of the peptides with model bacterial membranes comprising mixtures of the lipids DPPG [1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol] and DPPE [1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine], was studied using SAXS and cryogenic-TEM. Analysis of the SAXS structure factor indicates that R3F3 interacts with lipid bilayers by inducing correlation between bilayers, whereas R4F4 interacts with the bilayers causing an increase in polydispersity of the vesicle wall thickness. Both peptides break vesicles with a 1:3 DPPG:DPPE composition, which is close to the ratio of PG and PE lipids observed in the lipid membrane of Pseudomonas aeruginosa, a pathogen responsible for serious infections and which has developed antimicrobial resistant strains. Both peptides show activity against this bacterium in planktonic form. Peptide R4F4 shows particularly strong bioactivity against this microbe, with a minimum inhibitory concentration (MIC) value in the range of concentrations where the peptide is cytocompatible. It was further shown to have activity against other Pseudomonas species including the common plant pathogen Pseudomonas syringae. Finally, we show that R4F4 inhibits the development of P. aeruginosa biofilms. This was examined in detail and a proposed mechanism involving binding of the signaling molecule c-di-GMP is suggested, based on circular dichroism spectroscopy studies and Congo red assays of extracellular polysaccharides produced by the stressed bacteria. Thus, R4F4 is a promising candidate antimicrobial peptide with activity against Pseudomonas species.en
dc.description.versionPeer revieweden
dc.format.extent11
dc.format.extent1165-1175
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationEdwards-Gayle, C J C, Barrett, G, Roy, S, Castelletto, V, Seitsonen, J, Ruokolainen, J & Hamley, I W 2020, ' Selective Antibacterial Activity and Lipid Membrane Interactions of Arginine-Rich Amphiphilic Peptides ', ACS Applied Bio Materials, vol. 3, no. 2, pp. 1165-1175 . https://doi.org/10.1021/acsabm.9b00894en
dc.identifier.doi10.1021/acsabm.9b00894en_US
dc.identifier.issn2576-6422
dc.identifier.otherPURE UUID: edd633c4-9244-45b5-b82c-b4bf55724c09en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/edd633c4-9244-45b5-b82c-b4bf55724c09en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85080086363&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/53503846/Selective_Antibacterial_Activity.acsabm.9b00894.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/61830
dc.identifier.urnURN:NBN:fi:aalto-2020113020675
dc.language.isoenen
dc.publisherAMERICAN CHEMICAL SOCIETY
dc.relation.ispartofseriesACS Applied Bio Materialsen
dc.relation.ispartofseriesVolume 3, issue 2en
dc.rightsopenAccessen
dc.subject.keywordantimicrobialen_US
dc.subject.keywordliposomesen_US
dc.subject.keywordmodel membranesen_US
dc.subject.keywordpeptideen_US
dc.subject.keywordSAXSen_US
dc.subject.keywordself-assemblyen_US
dc.titleSelective Antibacterial Activity and Lipid Membrane Interactions of Arginine-Rich Amphiphilic Peptidesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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