Acrylamide/Alyssum campestre seed gum hydrogels enhanced with titanium carbide: Rheological insights for cardiac tissue engineering

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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en

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International Journal of Biological Macromolecules, Volume 293

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This study investigates the use of acrylamide and Alyssum campestre seed gum (ACSG) to create hydrogel composites with enhanced electrical and mechanical properties by incorporating titanium carbide (TiC). The composites were analyzed through techniques such as FTIR, SEM, TEM, TGA, swelling, rheology, tensile, electrical conductivity, antibacterial, and MTT assays. XRD analysis showed that 0.5 % TiC NPs were exfoliated in the hydrogel, while 1 % was intercalated. SEM images showed that ACSG created a semi-interpenetrating polymer network with interconnected cavities averaging 9.1 μm, which reduced to 3.6 μm with 0.5%TiC and 51.8 nm with 1%TiC due to increased crosslinking density. TGA results confirmed hydrogel stability at autoclave temperatures. Rheological testing revealed that the hydrogel exhibited a maximum resistance of 317 kPa. The addition of 1 % TiC enhanced its electrical conductivity to 1.5 × 10−2S/cm, making it suitable for applications in cardiac tissue engineering. MTT assays confirmed the hydrogel's biocompatibility and demonstrated its superior antibacterial activity against Staphylococcus aureus compared to Escherichia coli. The AM/ACSG/TiC hydrogel is a promising material for cardiac tissue engineering because of its adjustable mechanical properties, excellent electrical conductivity, and strong compatibility with cell cultures. The addition of ACSG improves the hydrogel's rheological behavior, which is crucial for promoting effective cell growth.

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Baniasadi, M, Baghban Salehi, M & Baniasadi, H 2025, 'Acrylamide/ Alyssum campestre seed gum hydrogels enhanced with titanium carbide: Rheological insights for cardiac tissue engineering', International Journal of Biological Macromolecules, vol. 293, 139240. https://doi.org/10.1016/j.ijbiomac.2024.139240