Toxicity Screening of Fungal Extracts and Metabolites, Xenobiotic Chemicals, and Indoor Dusts with In Vitro and Ex Vivo Bioassay Methods

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorHintikka, Tuomasen_US
dc.contributor.authorAndersson, Ainoen_US
dc.contributor.authorLundell, Tainaen_US
dc.contributor.authorMarik, Tamásen_US
dc.contributor.authorKredics, Laszloen_US
dc.contributor.authorMikkola, Raimoen_US
dc.contributor.authorAndersson, Magnus C.en_US
dc.contributor.authorKurnitski, Jareken_US
dc.contributor.authorSalonen, Heidien_US
dc.contributor.departmentDepartment of Civil Engineeringen
dc.contributor.groupauthorPerformance in Building Design and Constructionen
dc.contributor.organizationUniversity of Helsinkien_US
dc.contributor.organizationUniversity of Szegeden_US
dc.date.accessioned2024-03-27T07:55:29Z
dc.date.available2024-03-27T07:55:29Z
dc.date.issued2024-03en_US
dc.description.abstractIt is controversial how useful bioassays are for identifying the in vivo toxicity of hazardous environmental exposures. In this study, fruiting bodies of forest mushrooms (n = 46), indoor mold colonies (n = 412), fungal secondary metabolites (n = 18), xenobiotic chemicals such as biocides and detergents (n = 6), and methanol extracts of indoor dusts from urban buildings (n = 26) were screened with two different bioactivity assays: boar sperm motility inhibition (BSMI) and inhibition of cell proliferation (ICP) tests. For the forest mushrooms, the toxicity testing result was positive for 100% of poisonous-classified species, 69% of non-edible-classified species, and 18% of edible-classified species. Colonies of 21 isolates of Ascomycota mold fungal species previously isolated from water-damaged buildings proved to be toxic in the tests. Out of the fungal metabolites and xenobiotic chemicals, 94% and 100% were toxic, respectively. Out of the indoor dusts from moldy-classified houses (n = 12) and from dry, mold-free houses (n = 14), 50% and 57% were toxic, respectively. The bioassay tests, however, could not differentiate the samples from indoor dusts of moldy-classified buildings from those from the mold-free buildings. Xenobiotic chemicals and indoor dusts were more toxic in the BSMI assay than in the ICP assay, whereas the opposite results were obtained with the Ascomycota mold colonies and fungal secondary metabolites. The tests recognized unknown methanol-soluble thermoresistant substances in indoor settled dusts. Toxic indoor dusts may indicate a harmful exposure, regardless of whether the toxicity is due to xenobiotic chemicals or microbial metabolites.en
dc.description.versionPeer revieweden
dc.format.extent23
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationHintikka, T, Andersson, A, Lundell, T, Marik, T, Kredics, L, Mikkola, R, Andersson, M C, Kurnitski, J & Salonen, H 2024, ' Toxicity Screening of Fungal Extracts and Metabolites, Xenobiotic Chemicals, and Indoor Dusts with In Vitro and Ex Vivo Bioassay Methods ', Pathogens, vol. 13, no. 3, 217 . https://doi.org/10.3390/pathogens13030217en
dc.identifier.doi10.3390/pathogens13030217en_US
dc.identifier.issn2076-0817
dc.identifier.otherPURE UUID: 47a5a1fa-483b-4b0b-bb32-96627b186de1en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/47a5a1fa-483b-4b0b-bb32-96627b186de1en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85188912106&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/142024659/pathogens-13-00217-v2.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/127270
dc.identifier.urnURN:NBN:fi:aalto-202403272903
dc.language.isoenen
dc.publisherMDPI AG
dc.relation.ispartofseriesPathogens
dc.relation.ispartofseriesVolume 13, issue 3
dc.rightsopenAccessen
dc.subject.keywordAscomycetesen_US
dc.subject.keywordBasidiomycetesen_US
dc.subject.keywordBoar sperm motility inhibition assayen_US
dc.subject.keywordbioassaysen_US
dc.subject.keywordbiocidesen_US
dc.subject.keyworddetergentsen_US
dc.subject.keywordfungal metabolitesen_US
dc.subject.keywordindoor settled dusten_US
dc.subject.keywordinhibition of cell proliferation assayen_US
dc.titleToxicity Screening of Fungal Extracts and Metabolites, Xenobiotic Chemicals, and Indoor Dusts with In Vitro and Ex Vivo Bioassay Methodsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion

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