Indoor microbiota in severely moisture damaged homes and the impact of interventions

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorJayaprakash, Balamuralikrishna
dc.contributor.authorAdams, Rachel I.
dc.contributor.authorKirjavainen, Pirkka
dc.contributor.authorKarvonen, Anne
dc.contributor.authorVepsäläinen, Asko
dc.contributor.authorValkonen, Maria
dc.contributor.authorJarvi, Kati
dc.contributor.authorSulyok, Michael
dc.contributor.authorPekkanen, Juha
dc.contributor.authorHyvärinen, Anne
dc.contributor.authorTaubel, Martin
dc.contributor.departmentNational Institute for Health and Welfare
dc.contributor.departmentUniversity of California
dc.contributor.departmentDepartment of Civil Engineering
dc.contributor.departmentUniversity of Natural Resources and Life Sciences
dc.date.accessioned2017-11-21T13:37:43Z
dc.date.available2017-11-21T13:37:43Z
dc.date.issued2017-10-13
dc.description.abstractBackground: The limited understanding of microbial characteristics in moisture-damaged buildings impedes efforts to clarify which adverse health effects in the occupants are associated with the damage and to develop effective building intervention strategies. The objectives of this current study were (i) to characterize fungal and bacterial microbiota in house dust of severely moisture-damaged residences, (ii) to identify microbial taxa associated with moisture damage renovations, and (iii) to test whether the associations between the identified taxa and moisture damage are replicable in another cohort of homes. We applied bacterial 16S rRNA gene and fungal ITS amplicon sequencing complemented with quantitative PCR and chemical-analytical approaches to samples of house dust, and also performed traditional cultivation of bacteria and fungi from building material samples. Results: Active microbial growth on building materials had significant though small influence on the house dust bacterial and fungal communities. Moisture damage interventions-including actual renovation of damaged homes and cases where families moved to another home-had only a subtle effect on bacterial community structure, seen as shifts in abundance weighted bacterial profiles after intervention. While bacterial and fungal species richness were reduced in homes that were renovated, they were not reduced for families that moved houses. Using different discriminant analysis tools, we were able identify taxa that were significantly reduced in relative abundance during renovation of moisture damage. For bacteria, the majority of candidates belonged to different families within the Actinomycetales order. Results for fungi were overall less consistent. A replication study in approximately 400 homes highlighted some of the identified taxa, confirming associations with observations of moisture damage and mold. Conclusions: The present study is one of the first studies to analyze changes in microbiota due to moisture damage interventions using high-throughput sequencing. Our results suggest that effects of moisture damage and moisture damage interventions may appear as changes in the abundance of individual, less common, and especially bacterial taxa, rather than in overall community structure.en
dc.description.versionPeer revieweden
dc.format.extent17
dc.format.mimetypeapplication/pdf
dc.identifier.citationJayaprakash , B , Adams , R I , Kirjavainen , P , Karvonen , A , Vepsäläinen , A , Valkonen , M , Jarvi , K , Sulyok , M , Pekkanen , J , Hyvärinen , A & Taubel , M 2017 , ' Indoor microbiota in severely moisture damaged homes and the impact of interventions ' , MICROBIOME , vol. 5 , no. 1 , 138 . https://doi.org/10.1186/s40168-017-0356-5en
dc.identifier.doi10.1186/s40168-017-0356-5
dc.identifier.issn2049-2618
dc.identifier.otherPURE UUID: 960f044e-91ba-4509-9da5-bc616ce99f7b
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/960f044e-91ba-4509-9da5-bc616ce99f7b
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85049040739&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/15874984/s40168_017_0356_5.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/28830
dc.identifier.urnURN:NBN:fi:aalto-201711217651
dc.language.isoenen
dc.relation.ispartofseriesMicrobiomeen
dc.relation.ispartofseriesVolume 5en
dc.rightsopenAccessen
dc.subject.keywordIndoor
dc.subject.keywordBuilt environment
dc.subject.keywordMoisture damage
dc.subject.keywordDampness
dc.subject.keywordIndoor mold
dc.subject.keywordIntervention
dc.subject.keywordHouse dust microbiota
dc.subject.keywordAmplicon sequencing
dc.subject.keywordIN-HOUSE DUST
dc.subject.keywordBUILDING-MATERIALS
dc.subject.keywordRESPIRATORY HEALTH
dc.subject.keywordFUNGAL DIVERSITY
dc.subject.keywordBACTERIAL
dc.subject.keywordWATER
dc.subject.keywordAIR
dc.subject.keywordDAMPNESS
dc.subject.keywordMOLD
dc.subject.keywordSEQUENCES
dc.titleIndoor microbiota in severely moisture damaged homes and the impact of interventionsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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