Indoor microbiota in severely moisture damaged homes and the impact of interventions
dc.contributor | Aalto-yliopisto | fi |
dc.contributor | Aalto University | en |
dc.contributor.author | Jayaprakash, Balamuralikrishna | en_US |
dc.contributor.author | Adams, Rachel I. | en_US |
dc.contributor.author | Kirjavainen, Pirkka | en_US |
dc.contributor.author | Karvonen, Anne | en_US |
dc.contributor.author | Vepsäläinen, Asko | en_US |
dc.contributor.author | Valkonen, Maria | en_US |
dc.contributor.author | Jarvi, Kati | en_US |
dc.contributor.author | Sulyok, Michael | en_US |
dc.contributor.author | Pekkanen, Juha | en_US |
dc.contributor.author | Hyvärinen, Anne | en_US |
dc.contributor.author | Taubel, Martin | en_US |
dc.contributor.department | Department of Civil Engineering | en |
dc.contributor.organization | Finnish Institute for Health and Welfare (THL) | en_US |
dc.contributor.organization | University of California | en_US |
dc.contributor.organization | University of Natural Resources and Life Sciences, Vienna | en_US |
dc.date.accessioned | 2017-11-21T13:37:43Z | |
dc.date.available | 2017-11-21T13:37:43Z | |
dc.date.issued | 2017-10-13 | en_US |
dc.description.abstract | Background: The limited understanding of microbial characteristics in moisture-damaged buildings impedes efforts to clarify which adverse health effects in the occupants are associated with the damage and to develop effective building intervention strategies. The objectives of this current study were (i) to characterize fungal and bacterial microbiota in house dust of severely moisture-damaged residences, (ii) to identify microbial taxa associated with moisture damage renovations, and (iii) to test whether the associations between the identified taxa and moisture damage are replicable in another cohort of homes. We applied bacterial 16S rRNA gene and fungal ITS amplicon sequencing complemented with quantitative PCR and chemical-analytical approaches to samples of house dust, and also performed traditional cultivation of bacteria and fungi from building material samples. Results: Active microbial growth on building materials had significant though small influence on the house dust bacterial and fungal communities. Moisture damage interventions-including actual renovation of damaged homes and cases where families moved to another home-had only a subtle effect on bacterial community structure, seen as shifts in abundance weighted bacterial profiles after intervention. While bacterial and fungal species richness were reduced in homes that were renovated, they were not reduced for families that moved houses. Using different discriminant analysis tools, we were able identify taxa that were significantly reduced in relative abundance during renovation of moisture damage. For bacteria, the majority of candidates belonged to different families within the Actinomycetales order. Results for fungi were overall less consistent. A replication study in approximately 400 homes highlighted some of the identified taxa, confirming associations with observations of moisture damage and mold. Conclusions: The present study is one of the first studies to analyze changes in microbiota due to moisture damage interventions using high-throughput sequencing. Our results suggest that effects of moisture damage and moisture damage interventions may appear as changes in the abundance of individual, less common, and especially bacterial taxa, rather than in overall community structure. | en |
dc.description.version | Peer reviewed | en |
dc.format.extent | 17 | |
dc.format.mimetype | application/pdf | en_US |
dc.identifier.citation | Jayaprakash, B, Adams, R I, Kirjavainen, P, Karvonen, A, Vepsäläinen, A, Valkonen, M, Jarvi, K, Sulyok, M, Pekkanen, J, Hyvärinen, A & Taubel, M 2017, ' Indoor microbiota in severely moisture damaged homes and the impact of interventions ', MICROBIOME, vol. 5, no. 1, 138 . https://doi.org/10.1186/s40168-017-0356-5 | en |
dc.identifier.doi | 10.1186/s40168-017-0356-5 | en_US |
dc.identifier.issn | 2049-2618 | |
dc.identifier.other | PURE UUID: 960f044e-91ba-4509-9da5-bc616ce99f7b | en_US |
dc.identifier.other | PURE ITEMURL: https://research.aalto.fi/en/publications/960f044e-91ba-4509-9da5-bc616ce99f7b | en_US |
dc.identifier.other | PURE LINK: http://www.scopus.com/inward/record.url?scp=85049040739&partnerID=8YFLogxK | en_US |
dc.identifier.other | PURE FILEURL: https://research.aalto.fi/files/15874984/s40168_017_0356_5.pdf | en_US |
dc.identifier.uri | https://aaltodoc.aalto.fi/handle/123456789/28830 | |
dc.identifier.urn | URN:NBN:fi:aalto-201711217651 | |
dc.language.iso | en | en |
dc.relation.ispartofseries | Microbiome | en |
dc.relation.ispartofseries | Volume 5 | en |
dc.rights | openAccess | en |
dc.rights | CC BY | en_US |
dc.rights.copyright | The Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en_US |
dc.subject.keyword | Indoor | en_US |
dc.subject.keyword | Built environment | en_US |
dc.subject.keyword | Moisture damage | en_US |
dc.subject.keyword | Dampness | en_US |
dc.subject.keyword | Indoor mold | en_US |
dc.subject.keyword | Intervention | en_US |
dc.subject.keyword | House dust microbiota | en_US |
dc.subject.keyword | Amplicon sequencing | en_US |
dc.subject.keyword | IN-HOUSE DUST | en_US |
dc.subject.keyword | BUILDING-MATERIALS | en_US |
dc.subject.keyword | RESPIRATORY HEALTH | en_US |
dc.subject.keyword | FUNGAL DIVERSITY | en_US |
dc.subject.keyword | BACTERIAL | en_US |
dc.subject.keyword | WATER | en_US |
dc.subject.keyword | AIR | en_US |
dc.subject.keyword | DAMPNESS | en_US |
dc.subject.keyword | MOLD | en_US |
dc.subject.keyword | SEQUENCES | en_US |
dc.title | Indoor microbiota in severely moisture damaged homes and the impact of interventions | en |
dc.type | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä | fi |
dc.type.version | publishedVersion |