Indoor microbiota in severely moisture damaged homes and the impact of interventions

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorJayaprakash, Balamuralikrishnaen_US
dc.contributor.authorAdams, Rachel I.en_US
dc.contributor.authorKirjavainen, Pirkkaen_US
dc.contributor.authorKarvonen, Anneen_US
dc.contributor.authorVepsäläinen, Askoen_US
dc.contributor.authorValkonen, Mariaen_US
dc.contributor.authorJarvi, Katien_US
dc.contributor.authorSulyok, Michaelen_US
dc.contributor.authorPekkanen, Juhaen_US
dc.contributor.authorHyvärinen, Anneen_US
dc.contributor.authorTaubel, Martinen_US
dc.contributor.departmentDepartment of Civil Engineeringen
dc.contributor.organizationFinnish Institute for Health and Welfare (THL)en_US
dc.contributor.organizationUniversity of Californiaen_US
dc.contributor.organizationUniversity of Natural Resources and Life Sciences, Viennaen_US
dc.date.accessioned2017-11-21T13:37:43Z
dc.date.available2017-11-21T13:37:43Z
dc.date.issued2017-10-13en_US
dc.description.abstractBackground: The limited understanding of microbial characteristics in moisture-damaged buildings impedes efforts to clarify which adverse health effects in the occupants are associated with the damage and to develop effective building intervention strategies. The objectives of this current study were (i) to characterize fungal and bacterial microbiota in house dust of severely moisture-damaged residences, (ii) to identify microbial taxa associated with moisture damage renovations, and (iii) to test whether the associations between the identified taxa and moisture damage are replicable in another cohort of homes. We applied bacterial 16S rRNA gene and fungal ITS amplicon sequencing complemented with quantitative PCR and chemical-analytical approaches to samples of house dust, and also performed traditional cultivation of bacteria and fungi from building material samples. Results: Active microbial growth on building materials had significant though small influence on the house dust bacterial and fungal communities. Moisture damage interventions-including actual renovation of damaged homes and cases where families moved to another home-had only a subtle effect on bacterial community structure, seen as shifts in abundance weighted bacterial profiles after intervention. While bacterial and fungal species richness were reduced in homes that were renovated, they were not reduced for families that moved houses. Using different discriminant analysis tools, we were able identify taxa that were significantly reduced in relative abundance during renovation of moisture damage. For bacteria, the majority of candidates belonged to different families within the Actinomycetales order. Results for fungi were overall less consistent. A replication study in approximately 400 homes highlighted some of the identified taxa, confirming associations with observations of moisture damage and mold. Conclusions: The present study is one of the first studies to analyze changes in microbiota due to moisture damage interventions using high-throughput sequencing. Our results suggest that effects of moisture damage and moisture damage interventions may appear as changes in the abundance of individual, less common, and especially bacterial taxa, rather than in overall community structure.en
dc.description.versionPeer revieweden
dc.format.extent17
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationJayaprakash, B, Adams, R I, Kirjavainen, P, Karvonen, A, Vepsäläinen, A, Valkonen, M, Jarvi, K, Sulyok, M, Pekkanen, J, Hyvärinen, A & Taubel, M 2017, ' Indoor microbiota in severely moisture damaged homes and the impact of interventions ', MICROBIOME, vol. 5, no. 1, 138 . https://doi.org/10.1186/s40168-017-0356-5en
dc.identifier.doi10.1186/s40168-017-0356-5en_US
dc.identifier.issn2049-2618
dc.identifier.otherPURE UUID: 960f044e-91ba-4509-9da5-bc616ce99f7ben_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/960f044e-91ba-4509-9da5-bc616ce99f7ben_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85049040739&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/15874984/s40168_017_0356_5.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/28830
dc.identifier.urnURN:NBN:fi:aalto-201711217651
dc.language.isoenen
dc.relation.ispartofseriesMicrobiomeen
dc.relation.ispartofseriesVolume 5en
dc.rightsopenAccessen
dc.rightsCC BYen_US
dc.rights.copyrightThe Author(s). 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.subject.keywordIndooren_US
dc.subject.keywordBuilt environmenten_US
dc.subject.keywordMoisture damageen_US
dc.subject.keywordDampnessen_US
dc.subject.keywordIndoor molden_US
dc.subject.keywordInterventionen_US
dc.subject.keywordHouse dust microbiotaen_US
dc.subject.keywordAmplicon sequencingen_US
dc.subject.keywordIN-HOUSE DUSTen_US
dc.subject.keywordBUILDING-MATERIALSen_US
dc.subject.keywordRESPIRATORY HEALTHen_US
dc.subject.keywordFUNGAL DIVERSITYen_US
dc.subject.keywordBACTERIALen_US
dc.subject.keywordWATERen_US
dc.subject.keywordAIRen_US
dc.subject.keywordDAMPNESSen_US
dc.subject.keywordMOLDen_US
dc.subject.keywordSEQUENCESen_US
dc.titleIndoor microbiota in severely moisture damaged homes and the impact of interventionsen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion

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