The effect of water on the crystallization of phytosterols and phytostanols in organic solutions
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2025-01-01
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Language
en
Pages
11
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European Journal of Pharmaceutical Sciences, Volume 204
Abstract
Phytosterols (C29H50O), also known as plant sterols and stanols, are valuable biomolecules with a variety of applications in the pharma, food, and cosmetics industries. Phytosterols are typically manufactured from vegetable oil and tall oil feedstocks through a cooling crystallization process. Depending on the feedstock used, the composition regarding individual phytosterols and phytostanols (saturated analogs of phytosterols), also varies to a large extent. In the current research it was observed that by adding a small amount of water to the organic solvent [i.e., n(water)/n(acetone) of 0.17, n(water)/n(ethanol) of 0.13, and n(water)/n(ethyl acetate) of 0.10], the final phytosterol profile regarding phytosterol and phytostanol concentrations can be modified. This can be explained by the different solubility behavior of phytosterols and phytostanols in the studied solvent systems, based on experimental results obtained from transmissivity measurements. Phytostanols have surprisingly low solubility when compared to phytosterols in all the studied solvent systems. However, in the presence of water, phytosterol solubility decreased more compared to phytostanols. To the best of our knowledge, this is the first time that phytostanol solubility has been systematically studied. Moreover, phytosterol and phytostanol concentrations in a crystallized product with varying binary solvent systems containing water has not previously been reported. The measured experimental solubility data correlated well with the studied solubility models (van't Hoff, modified Apelblat, Buchowski-Ksiazaczak (λh), and polynomial equations). Understanding the solubility behavior of phytosterols and phytostanols allows to optimize the crystallization process itself toward a broader raw material selection, and better yield and quality in the production of phytosterols from plant-based raw materials. In addition, these findings can potentially be further utilized in phytosterol formulations for various applications.Description
Publisher Copyright: © 2024 The Author(s)
Keywords
Binary solvent, Cooling crystallization, Phytostanols, Phytosterols, Solubility, Tall oil
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Översti, P, Han, B, Kavakka, J, Torssell, S, Tirronen, E, Louhi-Kultanen, M & Oinas, P 2025, ' The effect of water on the crystallization of phytosterols and phytostanols in organic solutions ', European Journal of Pharmaceutical Sciences, vol. 204, 106956 . https://doi.org/10.1016/j.ejps.2024.106956