Serum Albumin-Peptide Conjugates for Simultaneous Heparin Binding and Detection
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Date
2019
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Language
en
Pages
21891-21899
Series
ACS Omega
Abstract
Heparin is a polysaccharide-based anticoagulant agent, which is widely used in surgery and blood transfusion. However, overdosage of heparin may cause severe side effects such as bleeding and low blood platelet count. Currently, there is only one clinically licensed antidote for heparin: Protamine sulfate, which is known to provoke adverse effects. In this work, we present a stable and biocompatible alternative for protamine sulfate that is based on serum albumin, which is conjugated with a variable number of heparin-binding peptides. The heparin-binding efficiency of the conjugates was evaluated with methylene blue displacement assay, dynamic light scattering, and anti-Xa assay. We found that multivalency of the peptides played a key role in the observed heparin-binding affinity and complex formation. The conjugates had low cytotoxicity and low hemolytic activity, indicating excellent biocompatibility. Furthermore, a sensitive DNA competition assay for heparin detection was developed. The detection limit of heparin was 0.1 IU/mL, which is well below its therapeutic range (0.2-0.4 IU/mL). Such biomolecule-based systems are urgently needed for next-generation biocompatible materials capable of simultaneous heparin binding and sensing.Description
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Liu, Q, Välimäki, S, Shaukat, A, Shen, B, Linko, V & Kostiainen, M A 2019, ' Serum Albumin-Peptide Conjugates for Simultaneous Heparin Binding and Detection ', ACS Omega, vol. 4, no. 26, pp. 21891-21899 . https://doi.org/10.1021/acsomega.9b02883