Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

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Volume Title

A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Date

2019-03-05

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Language

en

Pages

1-8

Series

Journal of Visualized Experiments, issue 145

Abstract

The inherent addressability of DNA origami structures makes them ideal templates for the arrangement of metal nanoparticles into complex plasmonic nanostructures. The high spatial precision of a DNA origami-templated assembly allows controlling the coupling between plasmonic resonances of individual particles and enables tailoring optical properties of the constructed nanostructures. Recently, chiral plasmonic systems attracted a lot of attention due to the strong correlation between the spatial configuration of plasmonic assemblies and their optical responses (e.g., circular dichroism [CD]). In this protocol, we describe the whole workflow for the generation of DNA origami-based chiral assemblies of gold nanorods (AuNRs). The protocol includes a detailed description of the design principles and experimental procedures for the fabrication of DNA origami templates, the synthesis of AuNRs, and the assembly of origami-AuNR structures. In addition, the characterization of structures using transmission electron microscopy (TEM) and CD spectroscopy is included. The described protocol is not limited to chiral configurations and can be adapted for the construction of various plasmonic architectures.

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| openaire: EC/H2020/713645/EU//BioMEP

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Citation

Huang, Y, Nguyen, K & Kuzyk, A 2019, ' Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates ', Journal of Visualized Experiments, vol. 145, no. 145, e59280, pp. 1-8 . https://doi.org/10.3791/59280