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Monitoring and controlling of recombinant protein production with Pichia pastoris

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Kemiantekniikan korkeakoulu | Bachelor's thesis
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CHEM3054

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en

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29+5

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Proteins and peptides are the fundamental of the biopharmaceutical industry. Hundreds of drug products from them are currently circulated on the market, and many are under clinical trials. However, the complexity of proteins structure and function creates difficult for developing a new product or improving existing ones. Therefore, recombinant proteins, synthesised in a microorganism host cell, attract the attention of the industry because of their high yields and simplicity in the production process. Fermentation strategies are constantly reviewed and adjusted to maintain optimal conditions for maximum productivity and quality while minimising disadvantaged factors. This paper discusses in detail Pichia pastoris host system and different strategies to monitor and control the production process.

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Hummel, Michael

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Eerikäinen, Tero

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