Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorNugroho, Robertus Wahyu N.
dc.contributor.authorHarjumäki, Riina
dc.contributor.authorZhang, Xue
dc.contributor.authorLou, Yan Ru
dc.contributor.authorYliperttula, Marjo
dc.contributor.authorValle-Delgado, Juan José
dc.contributor.authorÖsterberg, Monika
dc.contributor.departmentDepartment of Bioproducts and Biosystems
dc.contributor.departmentUniversity of Helsinki
dc.date.accessioned2018-11-09T13:05:02Z
dc.date.available2018-11-09T13:05:02Z
dc.date.issued2019-01-01
dc.description.abstractBiomaterials of different nature have been and are widely studied for various biomedical applications. In many cases, biomaterial assemblies are designed to mimic biological systems. Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available. That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications. This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering. Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique. Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films. The adhesion between biomaterials films increased with the time that the films were kept in contact. The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time. In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films. Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed. These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.en
dc.description.versionPeer revieweden
dc.format.extent10
dc.format.extent571-580
dc.format.mimetypeapplication/pdf
dc.identifier.citationNugroho , R W N , Harjumäki , R , Zhang , X , Lou , Y R , Yliperttula , M , Valle-Delgado , J J & Österberg , M 2019 , ' Quantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopy ' , Colloids and Surfaces B: Biointerfaces , vol. 173 , pp. 571-580 . https://doi.org/10.1016/j.colsurfb.2018.09.073en
dc.identifier.doi10.1016/j.colsurfb.2018.09.073
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.otherPURE UUID: 28368293-9d0b-4ab9-9167-52e745b54595
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/28368293-9d0b-4ab9-9167-52e745b54595
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85055033323&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/32536046/CHEM_Nugroho_et_al_Quantifying_the_interactions_2019_Colloids_and_Surfaces_B.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/34587
dc.identifier.urnURN:NBN:fi:aalto-201811095628
dc.language.isoenen
dc.relation.ispartofseriesColloids and Surfaces B: Biointerfacesen
dc.relation.ispartofseriesVolume 173en
dc.rightsopenAccessen
dc.subject.keywordAdhesion
dc.subject.keywordAFM-colloidal probe technique
dc.subject.keywordCellulose nanofibrils
dc.subject.keywordCollagen
dc.subject.keywordLaminin
dc.subject.keywordSurface forces
dc.subject.keywordMODEL
dc.subject.keywordPROTEOGLYCAN
dc.subject.keywordMECHANICAL-PROPERTIES
dc.subject.keywordATOMIC-FORCE MICROSCOPE
dc.subject.keywordHYDROGEL
dc.subject.keywordMOLECULES
dc.subject.keywordORGANIZATION
dc.subject.keywordFILMS
dc.subject.keywordMICROENVIRONMENT
dc.subject.keywordCELL
dc.titleQuantifying the interactions between biomimetic biomaterials – collagen I, collagen IV, laminin 521 and cellulose nanofibrils – by colloidal probe microscopyen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
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