Beyond the Surface: A Methodological Exploration of Enzyme Impact along the Cellulose Fiber Cross-Section
Loading...
Access rights
openAccess
URL
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Other link related to publication (opens in new window)
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Other link related to publication (opens in new window)
Date
2024-05-13
Department
Major/Subject
Mcode
Degree programme
Language
en
Pages
11
Series
Biomacromolecules, Volume 25, issue 5, pp. 3076-3086
Abstract
Despite the wide range of analytical tools available for the characterization of cellulose, the in-depth characterization of inhomogeneous, layered cellulose fiber structures remains a challenge. When treating fibers or spinning man-made fibers, the question always arises as to whether the changes in the fiber structure affect only the surface or the entire fiber. Here, we developed an analysis tool based on the sequential limited dissolution of cellulose fiber layers. The method can reveal potential differences in fiber properties along the cross-sectional profile of natural or man-made cellulose fibers. In this analytical approach, carbonyl groups are labeled with a carbonyl selective fluorescence label (CCOA), after which thin fiber layers are sequentially dissolved with the solvent system DMAc/LiCl (9% w/v) and analyzed with size exclusion chromatography coupled with light scattering and fluorescence detection. The analysis of these fractions allowed for the recording of the changes in the chemical structure across the layers, resulting in a detailed cross-sectional profile of the different functionalities and molecular weight distributions. The method was optimized and tested in practice with LPMO (lytic polysaccharide monooxygenase)-treated cotton fibers, where it revealed the depth of fiber modification by the enzyme.Description
Publisher Copyright: © 2024 The Authors. Published by American Chemical Society.
Keywords
Other note
Citation
Sulaeva, I, Sto̷pamo, F G, Melikhov, I, Budischowsky, D, Rahikainen, J L, Borisova, A, Marjamaa, K, Kruus, K, Eijsink, V G H, Várnai, A & Potthast, A 2024, ' Beyond the Surface: A Methodological Exploration of Enzyme Impact along the Cellulose Fiber Cross-Section ', Biomacromolecules, vol. 25, no. 5, pp. 3076-3086 . https://doi.org/10.1021/acs.biomac.4c00152