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Supercharged Fluorescent Protein-Apoferritin Cocrystals for Lighting Applications
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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10
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ACS Nano, Volume 17, issue 21, pp. 21206-21215
Abstract
The application of fluorescent proteins (FPs) in optoelectronics is hindered by the need for effective protocols to stabilize them under device preparation and operational conditions. Factors such as high temperatures, irradiation, and organic solvent exposure contribute to the denaturation of FPs, resulting in a low device performance. Herein, we focus on addressing the photoinduced heat generation associated with FP motion and rapid heat transfer. This leads to device temperatures of approximately 65 °C, causing FP-denaturation and a subsequent loss of device functionality. We present a FP stabilization strategy involving the integration of electrostatically self-assembled FP-apoferritin cocrystals within a silicone-based color down-converting filter. Three key achievements characterize this approach: (i) an engineering strategy to design positively supercharged FPs (+22) without compromising photoluminescence and thermal stability compared to their native form, (ii) a carefully developed crystallization protocol resulting in highly emissive cocrystals that retain the essential photoluminescence features of the FPs, and (iii) a strong reduction of the device's working temperature to 40 °C, leading to a 40-fold increase in Bio-HLEDs stability compared to reference devices.
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| openaire: EC/H2020/101002258/EU//ProCrystal This work has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant Agreement No. 816856 (R.D.C.) and No. 101002258 (M.A.K.)). R.D.C. and J.B.V. acknowledge the European Union’s Horizon 2020 research for the MSCA grant AnBioLED No 101064305. M.N. and R.D.C. acknowledge FPNP-BioLED No. 101022975 funded by H2020-MSCA-IF-2020 of the European Commission. E.A.-P. acknowledges funding from Academy of Finland (Grant Agreement No. 341057). A.S., J.V.I.T., E.A.-P., and M.A.K. acknowledge the provision of facilities and technical support by Aalto University Bioeconomy Facilities and OtaNano-Nanomicroscopy Center (Aalto-NMC). The work was carried out under the Academy of Finland Centers of Excellece Programme (2022–2029) in Life-Inspired Hybrid Materials (LIBER), project numbers 346110 and 346112. We would like to acknowledge the Aalto University Internal Funding Call for Co-Operation initiatives with TUM.
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Patrian, M, Shaukat, A, Nieddu, M, Banda-Vázquez, J A, Timonen, J V I, Fuenzalida Werner, J P, Anaya-Plaza, E, Kostiainen, M A & Costa, R D 2023, 'Supercharged Fluorescent Protein-Apoferritin Cocrystals for Lighting Applications', ACS Nano, vol. 17, no. 21, pp. 21206-21215. https://doi.org/10.1021/acsnano.3c05284