Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2012
Major/Subject
Mcode
Degree programme
Language
en
Pages
8
291-298
Series
JOURNAL OF CONTROLLED RELEASE, Volume 164, issue 3
Abstract
Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components or added bioactive components are preferred from the immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived from the abundant plant sources provide the desired functionalities. We show 1) rheological properties that allow formation of a 3D scaffold in-situ after facile injection, 2) cellular biocompatibility without added growth factors, 3) cellular polarization, and 4) differentiation of human hepatic cell lines HepaRG and HepG2. At high shear stress, the aqueous NFC has small viscosity that supports injectability, whereas at low shear stress conditions the material is converted to an elastic gel. Due to the inherent biocompatibility without any additives, we conclude that NFC generates a feasible and sustained microenvironment for 3D cell culture for potential applications, such as drug and chemical testing, tissue engineering, and cell therapy.
Description
Keywords
Nanocellulose, 3D cell culture, Hepatocyte, Nanofiber, Plant-derived cellulose
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Citation
Bhattacharya, M, Malinen, M M, Lauren, P, Lou, Y-R, Kuisma, S W, Kanninen, L, Lille, M, Corlu, A, GuGuen-Guillouzo, C, Ikkala, O, Laukkanen, A, Urtti, A & Yliperttula, M 2012, ' Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture ', Journal of Controlled Release, vol. 164, no. 3, pp. 291-298 . https://doi.org/10.1016/j.jconrel.2012.06.039