Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Bioresource Technology, Volume 394
Microbial expansin-related proteins, including loosenins, can disrupt cellulose networks and increase enzyme accessibility to cellulosic substrates. Herein, four loosenins from Phanerochaete carnosa (PcaLOOLs), and a PcaLOOL fused to a family 63 carbohydrate-binding module, were compared for ability to boost the cellulolytic deconstruction of steam pretreated softwood (SSW) and kraft pulps from softwood (ND-BSKP) and hardwood (ND-BHKP). Amending the Cellic® CTec-2 cellulase cocktail with PcaLOOLs increased reducing products from SSW by up to 40 %, corresponding to 28 % higher glucose yield. Amending Cellic® CTec-2 with PcaLOOLs also increased the release of glucose from ND-BSKP and ND-BHKP by 82 % and 28 %, respectively. Xylose release from ND-BSKP and ND-BHKP increased by 47 % and 57 %, respectively, highlighting the potential of PcaLOOLs to enhance hemicellulose recovery. Scanning electron microscopy and fiber image analysis revealed fibrillation and curlation of ND-BSKP after PcaLOOL treatment, consistent with increasing enzyme accessibility to targeted substrates.
| openaire: EC/H2020/964764/EU//BioUPGRADE Funding Information: This project received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 964764 and Business Finland under grant agreement 7655/31/2019. The content presented in this document represents the views of the authors and the Commission is not responsible for any use that may be made of the information it contains. T.K. was supported by the Jenny and Antti Wihuri Foundation (Centre for Young Synbio Scientists). This work has made use of Aalto University’s Bioeconomy facilities. Publisher Copyright: © 2023 The Author(s)
Bioprocessing, Carbohydrate-active enzymes, Lignocellulose, Loosenins, Microbial expansins
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Dahiya, D, Koitto, T, Kutvonen, K, Wang, Y, Haddad Momeni, M, de Ruijter, S & Master, E R 2024, ' Fungal loosenin-like proteins boost the cellulolytic enzyme conversion of pretreated wood fiber and cellulosic pulps ', Bioresource Technology, vol. 394, 130188 .