Seasonal dynamics in structural characteristics within bark stems of cultivated willow (Salix sp.) by NMR and time-gated Raman spectroscopy

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorDou, Jinze
dc.contributor.authorKögler, Martin
dc.contributor.authorKesari, Kavindra Kumar
dc.contributor.authorPitkänen, Leena
dc.contributor.authorVuorinen, Tapani
dc.contributor.departmentDepartment of Bioproducts and Biosystems
dc.contributor.departmentVTT Technical Research Centre of Finland
dc.date.accessioned2023-03-15T07:10:40Z
dc.date.available2023-03-15T07:10:40Z
dc.date.issued2023-03-07
dc.descriptionFunding Information: This work also made use of the RawMatTERS Finland infrastructure (RAMI) facilities based at Aalto University. This work was a part of the Academy of Finland's Flagship Programme under project no. 318890 and no. 318891 (Competence Center for Materials Bioeconomy, FinnCERES). VTT Finland was supported by the Academy of Finland, provided through an Academy of Finland Flagship Programme (PREIN) on Photonics Research and Innovation, PREIN, no. 320165. The authors thank the Sensing Solutions research area of VTT for their support with the spectroscopic equipment and measurement time.
dc.description.abstractThe present study measures the seasonal dynamics of the chemical characteristics of pectin, hemicellulose, starch, protein, and soluble carbohydrates in the tissues of Scandinavian willow bark, which were examined during a complete annual cycle. Wet-chemical purification and non-destructive time-gated (TG) Raman spectroscopy were applied to illustrate the chemical characteristics as a response to the seasonal effect. Starch was found in a more complex linkage connection with hemicellulose and pectin in non-winter seasons, which is also supported by TG Raman spectroscopy. Interestingly, TG Raman spectroscopy also revealed that protein is more prominent in winter-harvested willow bark. The most striking seasonal effect investigated was in the structural characteristics of pectin. The pectin of winter-harvested willow bark has a larger proportion of the highly branched rhamnogalacturonan I (RG-I) domain compared to non-winter-harvested pectin. The study aims to build on this structural knowledge to provide seasonal-tailored enzymes and treat willow bark biologically so that sclerenchyma fibre bundles can be isolated. We demonstrate here for the first time that a combination of non-destructive TG Raman and wet-chemical purification identification methods provides more knowledge about the compositional changes throughout the seasonal variation of compounds in willow bark. The correlation between the seasonal dynamics and the structure of pectin from willow bark is discussed for the first time, which provides the essential knowledge required for optimizing the microbial consortium in valorizing willow bark that can possibly be harvested from multiple seasons.en
dc.description.versionPeer revieweden
dc.format.extent1908-1919
dc.format.mimetypeapplication/pdf
dc.identifier.citationDou , J , Kögler , M , Kesari , K K , Pitkänen , L & Vuorinen , T 2023 , ' Seasonal dynamics in structural characteristics within bark stems of cultivated willow ( Salix sp.) by NMR and time-gated Raman spectroscopy ' , Green Chemistry , vol. 25 , no. 5 , pp. 1908-1919 . https://doi.org/10.1039/d2gc04476een
dc.identifier.doi10.1039/d2gc04476e
dc.identifier.issn1463-9262
dc.identifier.issn1463-9270
dc.identifier.otherPURE UUID: f3808200-6d84-4a09-8f1d-62eb88633371
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/f3808200-6d84-4a09-8f1d-62eb88633371
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85148707549&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/102602441/CHEM_Dou_et_al_Seasonal_dynamics_2023_Green_Chemistry.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/120110
dc.identifier.urnURN:NBN:fi:aalto-202303152436
dc.language.isoenen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofseriesGreen Chemistryen
dc.relation.ispartofseriesVolume 25, issue 5en
dc.rightsopenAccessen
dc.titleSeasonal dynamics in structural characteristics within bark stems of cultivated willow (Salix sp.) by NMR and time-gated Raman spectroscopyen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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