DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Journal of Visualized Experiments, issue 151
Structural DNA nanotechnology provides a viable route for building from the bottom-up using DNA as construction material. The most common DNA nanofabrication technique is called DNA origami, and it allows high-throughput synthesis of accurate and highly versatile structures with nanometer-level precision. Here, it is shown how the spatial information of DNA origami can be transferred to metallic nanostructures by combining the bottom-up DNA origami with the conventionally used top-down lithography approaches. This allows fabrication of billions of tiny nanostructures in one step onto selected substrates. The method is demonstrated using bowtie DNA origami to create metallic bowtie-shaped antenna structures on silicon nitride or sapphire substrates. The method relies on the selective growth of a silicon oxide layer on top of the origami deposition substrate, thus resulting in a patterning mask for following lithographic steps. These nanostructure-equipped surfaces can be further used as molecular sensors (e.g., surface-enhanced Raman spectroscopy (SERS)) and in various other optical applications at the visible wavelength range owing to the small feature sizes (sub-10 nm). The technique can be extended to other materials through methodological modifications; therefore, the resulting optically active surfaces may find use in development of metamaterials and metasurfaces.
Piskunen , P , Shen , B , Julin , S , Ijäs , H , Toppari , J J , Kostiainen , M A & Linko , V 2019 , ' DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications ' , Journal of Visualized Experiments , no. 151 , e60313 . https://doi.org/10.3791/60313