aalto1 untyped-item.component.html
Lignans in knotwood of Norway spruce: Localisation with soft x-ray microscopy and scanning transmission electron microscopy with energy dispersive x-ray spectroscopy
Loading...
Access rights
openAccess
publishedVersion
URL
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
This publication is imported from Aalto University research portal.
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
View publication in the Research portal (opens in new window)
View/Open full text file from the Research portal (opens in new window)
Unless otherwise stated, all rights belong to the author. You may download, display and print this publication for Your own personal use. Commercial use is prohibited.
Date
Department
Major/Subject
Mcode
Degree programme
Language
en
Pages
22
Series
Molecules, Volume 25, issue 13, pp. 1-22
Abstract
Lignans are bioactive compounds that are especially abundant in the Norway spruce (Picea abies L. Karst.) knotwood. By combining a variety of chromatographic, spectroscopic and imaging techniques, we were able to quantify, qualify and localise the easily extractable lignans in the xylem tissue. The knotwood samples contained 15 different lignans according to the gas chromatography-mass spectrometry analysis. They comprised 16% of the knotwood dry weight and 82% of the acetone extract. The main lignans were found to be hydroxymatairesinols HMR1 and HMR2. Cryosectioned and resin-embedded ultrathin sections of the knotwood were analysed with scanning transmission X-ray microscopy (STXM). Cryosectioning was found to retain only lignan residues inside the cell lumina. In the resin-embedded samples, lignan was interpreted to be unevenly distributed inside the cell lumina, and partially confined in deposits which were either readily present in the lumina or formed when OsO4 used in staining reacted with the lignans. Furthermore, the multi-technique characterisation enabled us to obtain information on the chemical composition of the structural components of knotwood. A simple spectral analysis of the STXM data gave consistent results with the gas chromatographic methods about the relative amounts of cell wall components (lignin and polysaccharides). The STXM analysis also indicated that a torus of a bordered pit contained aromatic compounds, possibly lignin.
Description
| openaire: EC/H2020/730872/EU//CALIPSOplus
Other note
Citation
Mansikkala, T, Patanen, M, Kärkönen, A, Korpinen, R, Pranovich, A, Ohigashi, T, Swaraj, S, Seitsonen, J, Ruokolainen, J, Huttula, M, Saranpää, P & Piispanen, R 2020, 'Lignans in knotwood of Norway spruce : Localisation with soft x-ray microscopy and scanning transmission electron microscopy with energy dispersive x-ray spectroscopy', Molecules, vol. 25, no. 13, 2997, pp. 1-22. https://doi.org/10.3390/molecules25132997