Modular protein architectures for pH-dependent interactions and switchable assembly of nanocellulose

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorVoutilainen, S.
dc.contributor.authorPaananen, Arja
dc.contributor.authorLille, Martina
dc.contributor.authorLinder, Markus B.
dc.contributor.departmentVTT Technical Research Centre of Finland
dc.contributor.departmentDepartment of Bioproducts and Biosystems
dc.date.accessioned2019-07-30T07:21:03Z
dc.date.available2019-07-30T07:21:03Z
dc.date.issued2019-09-15
dc.description.abstractProtein engineering shows a wide range of possibilities for designing properties in novel materials. Following inspiration from natural systems we have studied how combinations or duplications of protein modules can be used to engineer their interactions and achieve functional properties. Here we used cellulose binding modules (CBM) coupled to spider silk N-terminal domains that dimerize in a pH-sensitive manner. We showed how the pH-sensitive switching into dimers affected cellulose binding affinity in relation to covalent coupling between CBMs. Finally, we showed how the pH-sensitive coupling could be used to assemble cellulose nanofibers in a dynamic pH-dependent way. The work shows how novel proteins can be designed by linking functional domains from widely different sources and thereby achieve new functions in the self-assembly of nanoscale materials.en
dc.description.versionPeer revieweden
dc.format.extent7
dc.format.extent270-276
dc.format.mimetypeapplication/pdf
dc.identifier.citationVoutilainen , S , Paananen , A , Lille , M & Linder , M B 2019 , ' Modular protein architectures for pH-dependent interactions and switchable assembly of nanocellulose ' , International Journal of Biological Macromolecules , vol. 137 , pp. 270-276 . https://doi.org/10.1016/j.ijbiomac.2019.06.227en
dc.identifier.doi10.1016/j.ijbiomac.2019.06.227
dc.identifier.issn0141-8130
dc.identifier.otherPURE UUID: e0993777-d401-4982-942a-e4adccb17ddc
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/e0993777-d401-4982-942a-e4adccb17ddc
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85068262151&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/35343160/CHEM_Voutilainen_et_al_Modular_protein_architectures_2019_Int_Jour_Bio_Macro.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/39518
dc.identifier.urnURN:NBN:fi:aalto-201907304573
dc.language.isoenen
dc.publisherElsevier
dc.relation.ispartofseriesInternational Journal of Biological Macromoleculesen
dc.relation.ispartofseriesVolume 137en
dc.rightsopenAccessen
dc.titleModular protein architectures for pH-dependent interactions and switchable assembly of nanocelluloseen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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