Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation

Loading...
Thumbnail Image
Access rights
openAccess
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2020-10-19
Major/Subject
Mcode
Degree programme
Language
en
Pages
Series
Scientific Reports, Volume 10, issue 1
Abstract
Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp3 carbon backbone, embedded with a percolating network of sp2 carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation.
Description
Keywords
Other note
Citation
Ludwig, A, Kesaf, S, Heikkinen, J J, Sukhanova, T, Khakipoor, S, Molinari, F, Pellegrino, C, Kim, S I, Han, J G, Huttunen, H J, Lauri, S E, Franssila, S, Jokinen, V & Rivera, C 2020, ' Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation ', Scientific Reports, vol. 10, no. 1, 17661 . https://doi.org/10.1038/s41598-020-74535-6