The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorRaji, Olanrewajuen_US
dc.contributor.authorArnling Bååth, Jennyen_US
dc.contributor.authorVuong, Thu V.en_US
dc.contributor.authorLarsbrink, Johanen_US
dc.contributor.authorOlsson, Lisbethen_US
dc.contributor.authorMaster, Emma R.en_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorProtein Technologyen
dc.contributor.organizationUniversity of Torontoen_US
dc.contributor.organizationChalmers University of Technologyen_US
dc.date.accessioned2021-01-25T10:11:21Z
dc.date.available2021-01-25T10:11:21Z
dc.date.issued2021-02en_US
dc.description| openaire: EC/H2020/648925/EU//BHIVE
dc.description.abstractGlucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.en
dc.description.versionPeer revieweden
dc.format.extent9
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationRaji, O, Arnling Bååth, J, Vuong, T V, Larsbrink, J, Olsson, L & Master, E R 2021, ' The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes ', FEBS Letters, vol. 595, no. 3, pp. 351-359 . https://doi.org/10.1002/1873-3468.14019en
dc.identifier.doi10.1002/1873-3468.14019en_US
dc.identifier.issn0014-5793
dc.identifier.otherPURE UUID: 4d7fddb3-e2c4-47f2-a8dd-cdd52b40feffen_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/4d7fddb3-e2c4-47f2-a8dd-cdd52b40feffen_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85099032360&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/55239016/1873_3468.14019.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/102141
dc.identifier.urnURN:NBN:fi:aalto-202101251451
dc.language.isoenen
dc.publisherElsevier Science B.V.
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/648925/EU//BHIVEen_US
dc.relation.ispartofseriesFEBS Lettersen
dc.rightsopenAccessen
dc.subject.keyword4-O-methyl d-glucuronic aciden_US
dc.subject.keywordcarbohydrate-active enzymesen_US
dc.subject.keywordenzyme adsorptionen_US
dc.subject.keywordglucuronoxylanen_US
dc.subject.keywordhemicellulasesen_US
dc.subject.keywordlignin–carbohydrate complexesen_US
dc.titleThe coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexesen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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