The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2021-02
Major/Subject
Mcode
Degree programme
Language
en
Pages
9
Series
FEBS Letters
Abstract
Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.
Description
| openaire: EC/H2020/648925/EU//BHIVE
Keywords
4-O-methyl d-glucuronic acid, carbohydrate-active enzymes, enzyme adsorption, glucuronoxylan, hemicellulases, lignin–carbohydrate complexes
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Citation
Raji , O , Arnling Bååth , J , Vuong , T V , Larsbrink , J , Olsson , L & Master , E R 2021 , ' The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes ' , FEBS Letters , vol. 595 , no. 3 , pp. 351-359 . https://doi.org/10.1002/1873-3468.14019