The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes
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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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en
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9
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FEBS Letters, Volume 595, issue 3, pp. 351-359
Abstract
Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.Description
| openaire: EC/H2020/648925/EU//BHIVE
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Raji, O, Arnling Bååth, J, Vuong, T V, Larsbrink, J, Olsson, L & Master, E R 2021, 'The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes', FEBS Letters, vol. 595, no. 3, pp. 351-359. https://doi.org/10.1002/1873-3468.14019