Computer-aided production of scaffolded DNA nanostructures from flat sheet meshes

The use of DNA as a nanoscale construction material has been a rapidly developing field since the 1980s, in particular since the introduction of scaffolded DNA origami in 2006. Although software is available for DNA origami design, the user is generally limited to architectures where finding the scaffold path through the object is trivial. Herein, we demonstrate the automated conversion of arbitrary two-dimensional sheets in the form of digital meshes into scaffolded DNA nanostructures. We investigate the properties of DNA meshes based on three different internal frameworks in standard folding buffer and physiological salt buffers. We then employ the triangulated internal framework and produce four 2D structures with complex outlines and internal features. We demonstrate that this highly automated technique is capable of producing complex DNA nanostructures that fold with high yield to their programmed configurations, covering around 70 % more surface area than classic origami flat sheets.

Keywords

atomic force microscopy, DNA origami, DNA structures, molecular simulation

DOI

10.1002/anie.201602446

Citation

Benson , E , Mohammed , A , Bosco , A , Teixeira , A I , Orponen , P & Högberg , B 2016 , ' Computer-aided production of scaffolded DNA nanostructures from flat sheet meshes ' , Angewandte Chemie , vol. 55 , no. 31 , pp. 8869-8872 . https://doi.org/10.1002/anie.201602446

Permanent link to this item

https://urn.fi/URN:NBN:fi:aalto-201711217673

Collections

[article-cris] Perustieteiden korkeakoulu / SCI

Show all metadata

Computer-aided production of scaffolded DNA nanostructures from flat sheet meshes

Access rights

Journal Title

Journal ISSN

Volume Title

Author

Date

Department

Major/Subject

Mcode

Degree programme

Language

Pages

Series

Abstract

Description

Keywords

Other note

DOI

Citation

Permanent link to this item

Collections