Control of D-lactic acid content in P(LA-3HB) copolymer in the yeast Saccharomyces cerevisiae using a synthetic gene expression system

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorYlinen, Annaen_US
dc.contributor.authorSalusjärvi, Lauraen_US
dc.contributor.authorToivari, Mervien_US
dc.contributor.authorPenttilä, Merjaen_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorSynthetic Biologyen
dc.contributor.organizationVTT Technical Research Centre of Finlanden_US
dc.date.accessioned2022-06-08T06:13:13Z
dc.date.available2022-06-08T06:13:13Z
dc.date.issued2022-06en_US
dc.descriptionFunding Information: This work was supported by the Maj ja Tor Nesslingin säätiö [grant number 201800005 ]; Jenny and Antti Wihuri Foundation (for the Center for Young Synbio Scientists); and Suomen kulttuurirahasto [grant number 00201193 ]; and Suomen Akatemia [grant numbers 310191 ]. Publisher Copyright: © 2022 The Authors
dc.description.abstractThe fully biobased polyhydroxyalkanoate (PHA) polymers provide interesting alternatives for petrochemical derived plastic materials. The mechanical properties of some PHAs, including the common poly(3-hydroxybutyrate) (PHB), are limited, but tunable by addition of other monomers into the polymer chain. In this study we present a precise synthetic biology method to adjust lactate monomer fraction of a polymer by controlling the monomer formation in vivo at gene expression level, independent of cultivation conditions. We used the modified doxycycline-based Tet-On approach to adjust the expression of the stereospecific D-lactate dehydrogenase gene (ldhA) from Leuconostoc mesenteroides to control D-lactic acid formation in yeast Saccharomyces cerevisiae. The synthetic Tet-On transcription factor with a VP16 activation domain was continuously expressed and its binding to a synthetic promoter with eight transcription factor specific binding sites upstream of the ldhA gene was controlled with the doxycycline concentration in the media. The increase in doxycycline concentration correlated positively with ldhA expression, D-lactic acid production, poly(D-lactic acid) (PDLA) accumulation in vivo, and D-lactic acid content in the poly(D-lactate-co-3-hydroxybutyrate) P(LA-3HB) copolymer. We demonstrated that the D-lactic acid content of the P(LA-3HB) copolymer can be adjusted linearly from 6 mol% to 93 mol% in vivo in S. cerevisiae. These results highlight the power of controlling gene expression and monomer formation in the tuning of the polymer composition. In addition, we obtained 5.6% PDLA and 19% P(LA-3HB) of the cell dry weight (CDW), which are over two- and five-fold higher accumulation levels, respectively, than reported in the previous studies with yeast. We also compared two engineered PHA synthases and discovered that in S. cerevisiae the PHA synthase PhaC1437Ps6-19 produced P(LA-3HB) copolymers with lower D-lactic acid content, but with higher molecular weight, in comparison to the PHA synthase PhaC1Pre.en
dc.description.versionPeer revieweden
dc.format.extent10
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationYlinen, A, Salusjärvi, L, Toivari, M & Penttilä, M 2022, ' Control of D-lactic acid content in P(LA-3HB) copolymer in the yeast Saccharomyces cerevisiae using a synthetic gene expression system ', Metabolic Engineering Communications, vol. 14, e00199 . https://doi.org/10.1016/j.mec.2022.e00199en
dc.identifier.doi10.1016/j.mec.2022.e00199en_US
dc.identifier.issn2214-0301
dc.identifier.otherPURE UUID: a43cc358-2704-4f73-ae51-699ec7b9b2b9en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/a43cc358-2704-4f73-ae51-699ec7b9b2b9en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85129267237&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/83411361/CHEM_Ylinen_et_al_Control_of_D_lactic_acid_2022_Metabolic_Engineering_Communications.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/114788
dc.identifier.urnURN:NBN:fi:aalto-202206083631
dc.language.isoenen
dc.publisherElsevier
dc.relation.ispartofseriesMetabolic Engineering Communicationsen
dc.relation.ispartofseriesVolume 14en
dc.rightsopenAccessen
dc.subject.keywordCopolymeren_US
dc.subject.keywordDoxycyclineen_US
dc.subject.keywordGene expressionen_US
dc.subject.keywordPHAen_US
dc.subject.keywordTet-onen_US
dc.subject.keywordYeasten_US
dc.titleControl of D-lactic acid content in P(LA-3HB) copolymer in the yeast Saccharomyces cerevisiae using a synthetic gene expression systemen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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