Oat protein concentrates with improved solubility produced by an enzyme-aided ultrafiltration extraction method

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A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
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Foods, Volume 10, issue 12
The aim of this study was to develop an extraction method to produce highly functional oat protein concentrates. We investigated the possibility of combining enzyme-aided slightly alkaline (pH 8.0) extraction with ultrafiltration and subsequent diafiltration for concentration of the extracted oat proteins. A further aim was to study how the deamidation of oat proteins with protein-glutaminase (PG) improves the solubility of proteins as a function of the following parameters: pH (6.0–9.0), enzyme dosage (4–20 U/g protein), and incubation time (1–4 h) with response surface methodology (RSM). Furthermore, we investigated selected functional properties, such as heat-induced gelation and solubility, of the oat protein concentrates. The chosen parameters for the enzymatic deamidation pre-treatment process by PG were as follows: pH 8.0, dosage 11.0 U/g pro-tein, and an incubation time of 4 h (1 h at native pH and 3 h at pH 8.0). Two oat protein concentrates were produced, non-deamidated and ultrafiltered, and deamidated and ultrafiltered, with protein concentrations of 45.0 and 52.4%, respectively. The solubility of both oat protein concentrates was significantly improved at neutral and slightly alkaline pH compared to the solubility of proteins extracted from the starting material. Additionally, both oat protein concentrates produced equally strong heat-induced gel-like structures at a protein concentration of 10%.
Funding Information: Funding: This research was jointly funded by Valio Ltd., and Business Finland (Oat Abroad project 5955/31/2018). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Deamidation, Diafiltration, Heat-induced gelation, Oat protein concentrate, Protein-glutaminase, Ultrafiltration
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Immonen, M, Myllyviita, J, Sontag-Strohm, T & Myllärinen, P 2021, ' Oat protein concentrates with improved solubility produced by an enzyme-aided ultrafiltration extraction method ', Foods, vol. 10, no. 12, 3050 . https://doi.org/10.3390/foods10123050