Single-step fiber pretreatment with monocomponent endoglucanase : Defibrillation energy and cellulose nanofibril quality

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dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorBerto, Gabriela L.en_US
dc.contributor.authorMattos, Bruno D.en_US
dc.contributor.authorRojas, Orlando J.en_US
dc.contributor.authorArantes, Valdeiren_US
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.contributor.groupauthorBio-based Colloids and Materialsen
dc.contributor.organizationUniversidade de São Pauloen_US
dc.date.accessioned2021-02-26T07:11:02Z
dc.date.available2021-02-26T07:11:02Z
dc.date.embargoinfo:eu-repo/date/embargoEnd/2022-02-08en_US
dc.date.issued2021-02-08en_US
dc.description| openaire: EC/H2020/788489/EU//BioELCell
dc.description.abstractThe combination of enzymatic pretreatment of cellulose fibers followed by mechanical defibrillation has become a green and low-energy route to obtain cellulose nanofibrils (CNF). However, the variability in the properties of the as-produced CNF remains a major challenge that needs to be addressed for any application to be realized. Herein, we study the effect of monocomponent endoglucanase (EG) on the energy consumed in defibrillation as well as the physical properties of the obtained CNF. This single-step enzymatic pretreatment (0.5−25 EGU/g cellulose fibers for 1−3 h) reduces the defibrillation energy (by up to 50%) at nearly 100% yield to obtain CNF of a similar morphology, size, and crystallinity compared to CNF obtained in the absence of pretreatment. Under mild conditions (5.6 EGU/g for 1 h), aiming to minimize energy consumption while preserving rheological properties, EG pretreatment increased the water retention value, reduced the molecular weight, and promoted structural surface modification (amorphogenesis), without significant cellulose solubilization. In addition, the carbohydrate binding module of the EG was found to improve the interaction of the catalytic core with the substrate. The combination of the factors considered here boosts the effect of the enzyme even if used at low loadings, facilitating high-yield, more sustainable production of CNF.en
dc.description.versionPeer revieweden
dc.format.extent11
dc.format.extent2260-2270
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationBerto, G L, Mattos, B D, Rojas, O J & Arantes, V 2021, ' Single-step fiber pretreatment with monocomponent endoglucanase : Defibrillation energy and cellulose nanofibril quality ', ACS Sustainable Chemistry and Engineering, vol. 9, no. 5, pp. 2260-2270 . https://doi.org/10.1021/acssuschemeng.0c08162en
dc.identifier.doi10.1021/acssuschemeng.0c08162en_US
dc.identifier.issn2168-0485
dc.identifier.otherPURE UUID: 1302eb33-3631-4b85-a9a1-2074064a0189en_US
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/1302eb33-3631-4b85-a9a1-2074064a0189en_US
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85100278643&partnerID=8YFLogxKen_US
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/56086560/CHEM_Berto_et_al_Single_step_fiber_pre_treatment_ACS_Sustainable_Chemistry_and_Engineering.pdfen_US
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/102741
dc.identifier.urnURN:NBN:fi:aalto-202102262030
dc.language.isoenen
dc.publisherAMERICAN CHEMICAL SOCIETY
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/788489/EU//BioELCellen_US
dc.relation.ispartofseriesACS Sustainable Chemistry and Engineeringen
dc.relation.ispartofseriesVolume 9, issue 5en
dc.rightsopenAccessen
dc.subject.keywordEndoglucanasesen_US
dc.subject.keywordEnergy reductionen_US
dc.subject.keywordEnzyme−substrate interactionsen_US
dc.subject.keywordSingle-step pretreatmenten_US
dc.titleSingle-step fiber pretreatment with monocomponent endoglucanase : Defibrillation energy and cellulose nanofibril qualityen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
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[article-cris] Kemian tekniikan korkeakoulu / CHEM