Cellulose elementary fibril orientation in the spruce S1-2 transition layer

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Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2019-03-07
Major/Subject
Mcode
Degree programme
Language
en
Pages
1-7
Series
Scientific Reports, Volume 9, issue 1
Abstract
The tight organization of major wood cell wall polymers limits the swellability, solubility and reactivity of cellulose fibers during the production of regenerated textile fibers, nanocellulose, bioethanol, and many other value-added products. However, the ultrastructural assembly of cellulose elementary fibrils (EF) and matrix materials in one of the outer layers, i.e. S 1-2 transition layer of wood cell wall, is far from being understood. Here, single-axis electron tomography on ultrathin spruce sections was applied to observe the three-dimensional (3D) structure of the S 1-2 layer. The nanoscale geometries of the EFs were further quantitatively modeled through mathematical fitting of the tomographic subvolumes by suitable parametric space curves. The results showed that crisscross, bundled and parallel EF organizations are all present in this layer; the former two exhibit a denser structure. Several quantitative measures such as distances and angles were obtained for the analyzed structures. The result obtained in this study suggests that the S 1-2 transition layer differs in structure than the principal cell wall layers. The structural differences and its possible role in wood cell wall have been discussed. These results will enhance our understanding of the swellability, accessibility and solubility of woody biomass for its conversion into the aforementioned value-added products.
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Keywords
CELL-WALL, ELECTRON TOMOGRAPHY, FE-SEM, WOOD, APPLICABILITY, VISUALIZATION, MICROFIBRILS, ORGANIZATION, AGGREGATION, BIOMASS
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Citation
Reza, M, Bertinetto, C, Kesari, K K, Engelhardt, P, Ruokolainen, J & Vuorinen, T 2019, ' Cellulose elementary fibril orientation in the spruce S 1-2 transition layer ', Scientific Reports, vol. 9, no. 1, 3869, pp. 1-7 . https://doi.org/10.1038/s41598-019-40303-4