Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorCannazza, Pietro
dc.contributor.authorRissanen, Antti J.
dc.contributor.authorSarlin, Essi
dc.contributor.authorGuizelini, Dieval
dc.contributor.authorMinardi, Carlotta
dc.contributor.authorLosoi, Pauli
dc.contributor.authorMolinari, Francesco
dc.contributor.authorRomano, Diego
dc.contributor.authorMangayil, Rahul
dc.contributor.departmentUniversity of Milano
dc.contributor.departmentTampere University
dc.contributor.departmentUniversidade Federal do Paraná
dc.contributor.departmentDepartment of Bioproducts and Biosystems
dc.contributor.departmentDepartment of Bioproducts and Biosystemsen
dc.date.accessioned2022-12-22T09:45:21Z
dc.date.available2022-12-22T09:45:21Z
dc.date.issued2022-11-28
dc.descriptionFunding Information: R.M., A.J.R., and D.R. thanks Academy of Finland (Project No’s. 323214 and 346983), Kone Foundation (Project No. 201803224) and Fondazione Cariplo (2020–1070), respectively, for supporting this study. R.M. would like to thank Mr. Rohith Krishnan for providing the Kombucha SCOBY. Open access funding provided by Tampere University, Finland.
dc.description.abstractBacterial nanocellulose (BC) is a highly versatile biopolymer currently pursued as a material of choice in varied themes of biomedical and material science research fields. With the aim to extend the biotechnological applications, the genetic tractability of the BC producers within the Komagataeibacter genus and its potential as an alternative host chassis in synthetic biology have been extensively studied. However, such studies have been largely focused on the model Komagataeibacter spp. Here, we present a novel K. intermedius strain capable of utilizing glucose, and glycerol sources for biomass and BC synthesis. Genome assembly identified one bacterial cellulose synthetase (bcs) operon containing the complete gene set encoding the BC biogenesis machinery (bcsI) and three additional copies (bcsII–IV). Investigations on the genetic tractability confirmed plasmid transformation, propagation of vectors with pBBR1 and p15A origin of replications and constitutive and inducible induction of recombinant protein in K. intermedius ENS15. This study provides the first report on the genetic tractability of K. intermedius, serving as starting point towards future genetic engineering of this strain.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdf
dc.identifier.citationCannazza , P , Rissanen , A J , Sarlin , E , Guizelini , D , Minardi , C , Losoi , P , Molinari , F , Romano , D & Mangayil , R 2022 , ' Characterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolate ' , Scientific Reports , vol. 12 , no. 1 , 20520 . https://doi.org/10.1038/s41598-022-24735-zen
dc.identifier.doi10.1038/s41598-022-24735-z
dc.identifier.issn2045-2322
dc.identifier.otherPURE UUID: 8fa4868e-1489-4c14-b0b7-6dcd74564753
dc.identifier.otherPURE ITEMURL: https://research.aalto.fi/en/publications/8fa4868e-1489-4c14-b0b7-6dcd74564753
dc.identifier.otherPURE LINK: http://www.scopus.com/inward/record.url?scp=85142819127&partnerID=8YFLogxK
dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/95372866/CHEM_Cannazza_et_al_Characterization_genome_analysis_2022_Scientific_Reports.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/118520
dc.identifier.urnURN:NBN:fi:aalto-202212227258
dc.language.isoenen
dc.publisherNature Publishing Group
dc.relation.ispartofseriesScientific Reportsen
dc.relation.ispartofseriesVolume 12, issue 1en
dc.rightsopenAccessen
dc.titleCharacterization, genome analysis and genetic tractability studies of a new nanocellulose producing Komagataeibacter intermedius isolateen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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