A DNA origami-based device for investigating DNA bending proteins by transmission electron microscopy

dc.contributorAalto-yliopistofi
dc.contributorAalto Universityen
dc.contributor.authorNatarajan, Ashwin Karthick
dc.contributor.authorRyssy, Joonas
dc.contributor.authorKuzyk, Anton
dc.contributor.departmentDepartment of Neuroscience and Biomedical Engineering
dc.date.accessioned2023-02-08T07:35:50Z
dc.date.available2023-02-08T07:35:50Z
dc.date.issued2023-01-17
dc.description.abstractThe DNA origami technique offers precise positioning of nanoscale objects with high accuracy. This has facilitated the development of DNA origami-based functional nanomechanical devices that enable the investigation of DNA–protein interactions at the single particle level. Herein, we used the DNA origami technique to fabricate a nanoscale device for studying DNA bending proteins. For a proof of concept, we used TATA-box binding protein (TBP) to evaluate our approach. Upon binding to the TATA box, TBP causes a bend to DNA of ∼90°. Our device translates this bending into an angular change that is readily observable with a conventional transmission electron microscope (TEM). Furthermore, we investigated the roles of transcription factor II A (TF(II)A) and transcription factor II B (TF(II)B). Our results indicate that TF(II)A introduces additional bending, whereas TF(II)B does not significantly alter the TBP–DNA structure. Our approach can be readily adopted to a wide range of DNA-bending proteins and will aid the development of DNA-origami-based devices tailored for the investigation of DNA–protein interactions.en
dc.description.versionPeer revieweden
dc.format.mimetypeapplication/pdf
dc.identifier.citationNatarajan , A K , Ryssy , J & Kuzyk , A 2023 , ' A DNA origami-based device for investigating DNA bending proteins by transmission electron microscopy ' , Nanoscale , vol. 15 , no. 7 , pp. 3212-3218 . https://doi.org/10.1039/D2NR05366Gen
dc.identifier.doi10.1039/D2NR05366G
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.otherPURE UUID: 4f8a8ff9-5ec6-4246-a0cf-c6a7a718ef1d
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dc.identifier.otherPURE FILEURL: https://research.aalto.fi/files/99929750/A_DNA_origami_based_device_for_investigating_DNA_bending_proteins_by_transmission_electron_microscopy.pdf
dc.identifier.urihttps://aaltodoc.aalto.fi/handle/123456789/119674
dc.identifier.urnURN:NBN:fi:aalto-202302082024
dc.language.isoenen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofseriesNanoscaleen
dc.rightsopenAccessen
dc.titleA DNA origami-based device for investigating DNA bending proteins by transmission electron microscopyen
dc.typeA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessäfi
dc.type.versionpublishedVersion
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