A DNA origami-based device for investigating DNA bending proteins by transmission electron microscopy

Loading...
Thumbnail Image
Access rights
openAccess
Journal Title
Journal ISSN
Volume Title
A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä
Date
2023-01-17
Major/Subject
Mcode
Degree programme
Language
en
Pages
Series
Nanoscale
Abstract
The DNA origami technique offers precise positioning of nanoscale objects with high accuracy. This has facilitated the development of DNA origami-based functional nanomechanical devices that enable the investigation of DNA–protein interactions at the single particle level. Herein, we used the DNA origami technique to fabricate a nanoscale device for studying DNA bending proteins. For a proof of concept, we used TATA-box binding protein (TBP) to evaluate our approach. Upon binding to the TATA box, TBP causes a bend to DNA of ∼90°. Our device translates this bending into an angular change that is readily observable with a conventional transmission electron microscope (TEM). Furthermore, we investigated the roles of transcription factor II A (TF(II)A) and transcription factor II B (TF(II)B). Our results indicate that TF(II)A introduces additional bending, whereas TF(II)B does not significantly alter the TBP–DNA structure. Our approach can be readily adopted to a wide range of DNA-bending proteins and will aid the development of DNA-origami-based devices tailored for the investigation of DNA–protein interactions.
Description
Keywords
Other note
Citation
Natarajan, A K, Ryssy, J & Kuzyk, A 2023, ' A DNA origami-based device for investigating DNA bending proteins by transmission electron microscopy ', Nanoscale, vol. 15, no. 7, pp. 3212-3218 . https://doi.org/10.1039/D2NR05366G